Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China; State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China.
Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing 100035, PR China.
Colloids Surf B Biointerfaces. 2018 Oct 1;170:201-209. doi: 10.1016/j.colsurfb.2018.06.012. Epub 2018 Jun 15.
Due to the significant role of the periosteum in bone regeneration, we hypothesised that using a specially engineered artificial periosteum could lead to an enhancement in osteogenesis in bone grafts. Herein, we describe our work aimed at fabricating an electrospun fibrous membrane as an artificial periosteum that exhibits flexibility, permeability and osteoinduction. This membrane was designed to cover the complex surface of bone grafts to facilitate and accelerate bone regeneration. The traditional Chinese medicine icariin (ICA) was subsequently introduced into poly (ε-caprolactone) (PCL) /gelatin nanofibers to fabricate an artificial periosteum for the first time. The effects of ICA content on morphology, physical properties, drug release profile, in vitro degradability, biocompatibility and osteogenic differentiation properties were investigated. The ICA-loaded electrospun membranes showed significant improvement in hydrophilicity, high mechanical strength, appropriate degradation rates and excellent biocompatibility. Furthermore, clear enhancement in alkaline phosphatase (ALP) activity, as well as an increase in osteocalcin (OCN) and type collagen I (COL I) expression in MC3T3-E1 cells were detected. Furthermore, we observed clear calcium deposition content in MC3T3-E1 cells cultured on ICA-loaded fibrous matrix. The membrane loaded with 0.05 wt.% ICA displayed properties contributing to cell attachment, proliferation and differentiation. These results indicate the huge potential of this ICA-loaded PCL/gelatin electrospun membrane as a biomimetic artificial periosteum to accelerate bone regeneration.
由于骨膜在骨再生中的重要作用，我们假设使用专门设计的人工骨膜可以增强骨移植物的成骨作用。在此，我们描述了我们旨在制造一种电纺纤维膜作为人工骨膜的工作，该膜具有柔韧性、通透性和诱导成骨作用。该膜旨在覆盖骨移植物的复杂表面，以促进和加速骨再生。随后，我们首次将中药淫羊藿苷 (ICA) 引入聚 (ε-己内酯) (PCL)/明胶纳米纤维中，以制造人工骨膜。研究了 ICA 含量对形态、物理性能、药物释放曲线、体外降解性、生物相容性和成骨分化特性的影响。载 ICA 的电纺膜表现出明显的亲水性改善、高机械强度、适当的降解率和优异的生物相容性。此外，在 MC3T3-E1 细胞中检测到碱性磷酸酶 (ALP) 活性的明显增强，以及骨钙素 (OCN) 和 I 型胶原蛋白 (COL I) 表达的增加。此外，我们观察到在载 ICA 的纤维基质上培养的 MC3T3-E1 细胞中有明显的钙沉积含量。负载 0.05wt.%ICA 的膜具有促进细胞附着、增殖和分化的特性。这些结果表明，这种载 ICA 的 PCL/明胶电纺膜作为仿生人工骨膜具有巨大的加速骨再生的潜力。
