Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, PR China; State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, PR China.
Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing, PR China.
Nanomedicine. 2019 Apr;17:124-136. doi: 10.1016/j.nano.2019.01.002. Epub 2019 Jan 19.
The traditional Chinese medicine icariin (ICA) and broad-spectrum antibacterial drug moxifloxacin hydrochloride (MOX) were introduced into a polycaprolactone core and gelatin shell, respectively, to develop osteogenic and antibacterial biomimetic periosteum by coaxial electrospinning. The physical properties, drug release, degradation, antibacterial property, in vitro and in vivo osteogenesis performances were investigated. Results demonstrated that stepwise and controlled drug release profiles were achieved based on the core-shell configuration and disparate degradation rate of PCL and gelatin. Only 20% ICA was released from this dual drug-loaded membrane after 1 month while the release of MOX was almost completed. Moreover, clear in vitro antibacterial effect and enhancement in osteogenic marker expressions including osteocalcin, type-I collagen expression, and calcium deposition were observed. Notably, the dual drug-loaded membrane displayed fascinating properties contributing to in vivo bone formation in terms of quality and quantity in a rabbit radius defect model.
将中药淫羊藿苷（ICA）和广谱抗菌药盐酸莫西沙星（MOX）分别引入到聚己内酯核和明胶壳中，通过同轴静电纺丝技术开发出具有成骨和抗菌仿生特性的骨膜。研究了物理性能、药物释放、降解、抗菌性能、体外和体内成骨性能。结果表明，基于核壳结构和聚己内酯与明胶不同的降解速率，实现了分步和控制药物释放。在 1 个月后，只有 20%的 ICA 从这种双药物负载膜中释放出来，而 MOX 的释放几乎完成。此外，还观察到体外明显的抗菌效果以及成骨标志物表达（包括骨钙素、I 型胶原蛋白表达和钙沉积）的增强。值得注意的是，在兔桡骨缺损模型中，双药物负载膜在质量和数量方面都表现出了令人瞩目的促进体内骨形成的特性。
