Farzaneh Zahra, Kalantar Kurosh, Iraji Aida, Amirghofran Zahra
a Department of Immunology , Shiraz University of Medical Sciences , Shiraz , Iran.
b Central Research Laboratory , Shiraz University of Medical Sciences , Shiraz , Iran.
J Immunoassay Immunochem. 2018;39(3):274-291. doi: 10.1080/15321819.2018.1480495. Epub 2018 Jun 18.
Macrophages are among the main cells involved in generation of inflammation. To investigate the anti-inflammatory effect of Satureja hortensis (summer savory), lipopolysaccharide (LPS)-activated J774.1 macrophages were treated with various extracts, and the expression and release of various inflammatory molecules by macrophages were examined. We showed that dichloromethane and hexane extracts reduced nitric oxide (NO) production more efficiently than other extracts. Both extracts decreased gene expression of inducible NO synthase (iNOS) (<0.44 fold of control), cyclooxygenase (COX)-2 (<0.29 fold), interleukin (IL)-1β (<0.41 fold), IL-6 (<0.25 fold) and tumor necrosis factor (TNF)-α (<0.2 fold). The extracts reduced IL-6 and IL-1β proteins production from macrophages. Surface intensity of expression of intercellular adhesion molecule (ICAM)-1 decreased to 845 ± 28.1 (dichloromethane) and 715 ± 48.6 (hexane) compared to the control (902 ± 73.1). These findings showed that Satureja hortensis, by influencing macrophages and related mediators, could contribute to reduction of inflammation and might be useful as an anti-inflammatory agent.
巨噬细胞是参与炎症产生的主要细胞之一。为了研究夏香薄荷的抗炎作用，用各种提取物处理脂多糖（LPS）激活的J774.1巨噬细胞，并检测巨噬细胞中各种炎症分子的表达和释放。我们发现二氯甲烷和己烷提取物比其他提取物更有效地降低了一氧化氮（NO）的产生。两种提取物均降低了诱导型一氧化氮合酶（iNOS）（<对照的0.44倍）、环氧化酶（COX）-2（<0.29倍）、白细胞介素（IL）-1β（<0.41倍）、IL-6（<0.25倍）和肿瘤坏死因子（TNF）-α（<0.2倍）的基因表达。提取物减少了巨噬细胞中IL-6和IL-1β蛋白的产生。与对照（902±73.1）相比，细胞间粘附分子（ICAM）-1的表面表达强度降至845±28.1（二氯甲烷）和715±48.6（己烷）。这些发现表明，夏香薄荷通过影响巨噬细胞和相关介质，可能有助于减轻炎症，有望作为一种抗炎剂。
