Hjelt K, Grauballe P C, Nielsen O H, Schiøtz P O, Krasilnikoff P A
J Pediatr Gastroenterol Nutr. 1985 Jun;4(3):414-20. doi: 10.1097/00005176-198506000-00016.
The transfer of rotavirus antibodies from 25 healthy mothers to their breast-fed infants was investigated during the period of lactation (mean, 3.9 months; range, 1-9 months). Furthermore, the destiny of these antibodies in the infants' gastrointestinal tract and serum was examined. Rotavirus-specific immunoglobulins were analyzed by the ELISA (enzyme-linked immunosorbent assay) technique. All the mothers had rotavirus IgA and IgG in serum. About 80% of the mothers had low concentrations of rotavirus ScIg (i.e., antirotavirus immunoglobulin containing secretory component) in serum at the beginning of the lactation period declining to about 45% at the end of the period. From a few days after delivery to about 2 weeks later, the concentrations of rotavirus IgA and ScIg in milk declined. Thereafter, they remained unchanged. There was a positive correlation among the concentrations of rotavirus IgA in serum and rotavirus IgA as well as ScIg in milk. Rotavirus IgG in the infants' serum correlated with that of the mothers. Few samples of the infants' duodenal fluid contained rotavirus IgA or ScIg. On the other hand, about 80% of the infants' fecal samples contained rotavirus ScIg and IgA. Rotavirus IgA and ScIg disappeared from the infants' feces after cessation of lactation. Hence, it may be concluded that infants receive rotavirus IgG through the placenta, and rotavirus ScIg and IgA in constant amounts via milk throughout the period of lactation. The small intestine is flushed with rotavirus ScIg and IgA at each breast-meal, and these antibodies survive proteolysis in the gut. A possible protectional effect of rotavirus ScIg or IgA requires frequent breast-meals, and the effect is limited to the period of lactation.
在哺乳期（平均3.9个月；范围1 - 9个月）对25名健康母亲向其母乳喂养婴儿的轮状病毒抗体转移情况进行了调查。此外，还检测了这些抗体在婴儿胃肠道和血清中的去向。通过酶联免疫吸附测定（ELISA）技术分析轮状病毒特异性免疫球蛋白。所有母亲血清中均有轮状病毒IgA和IgG。约80%的母亲在哺乳期开始时血清中轮状病毒ScIg（即含分泌成分的抗轮状病毒免疫球蛋白）浓度较低，哺乳期结束时降至约45%。从分娩后几天到约2周后，母乳中轮状病毒IgA和ScIg浓度下降。此后，它们保持不变。血清中轮状病毒IgA浓度与母乳中轮状病毒IgA以及ScIg浓度之间存在正相关。婴儿血清中的轮状病毒IgG与母亲的相关。婴儿十二指肠液中很少有样本含有轮状病毒IgA或ScIg。另一方面，约80%的婴儿粪便样本含有轮状病毒ScIg和IgA。哺乳期结束后，轮状病毒IgA和ScIg从婴儿粪便中消失。因此，可以得出结论，婴儿在整个哺乳期通过胎盘接受轮状病毒IgG，并通过母乳持续获得轮状病毒ScIg和IgA。每次喂奶时，小肠都会被轮状病毒ScIg和IgA冲洗，这些抗体在肠道中能抵抗蛋白水解作用。轮状病毒ScIg或IgA可能的保护作用需要频繁喂奶，且这种作用仅限于哺乳期。
