Jantafong Tippawan, Ruenphet Sakchai, Punyadarsaniya Darsaniya, Takehara Kazuaki
Department of Immunology and Virology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Vet World. 2018 May;11(5):706-711. doi: 10.14202/vetworld.2018.706-711. Epub 2018 May 27.
The aim of this study was to determine the effectiveness of the fourth-generation quaternary ammonium compounds, didecyl dimethyl ammonium bromide (DDAB), on the efficacy of bacterial and viral decontamination against pathogens commonly found in livestock industry including infantis (SI), , and avian influenza virus (AIV).
The DDAB was prepared at 500, 250, and 125 parts per million (ppm) for absent and present organic material. Meanwhile, 5% of fetal bovine serum in DDAB solution sample was used to mimic the presence of organic material contamination. 400 µl of each DDAB concentration was mixed with 100 µl of each pathogen (SI, , and AIV) and then incubated at room temperature or 4°C at various time points (5 s, 30 s, 1 min, 5 min, 10 min, 15 min, and 30 min). The activity of DDAB treatment was stopped using 500 µl of FBS. Each treatment sample was titrated on either deoxycholate hydrogen sulfide lactose agar plates or Madin-Darby canine kidney cells for bacteria and AIV, respectively. Each treatment was conducted in triplicates, and the pathogen inactivation was considered effective when the reduction factor was ≥3 log.
Our current study revealed that the DDAB inactivated SI, , and AIV under the various concentrations of DDAB, organic material conditions, exposure temperature, and exposure timing. In addition, the comparison of bactericidal and virucidal efficacy indicated that bacteria were more susceptible to be inactivated by DDAB as compared to viruses. However, DDAB showed marked inactivated differences in the absence or presence of organic materials.
The DDAB may be a potential disinfectant for inactivating bacteria and viruses, especially enveloped viruses, in livestock farms. It can be useful as a disinfectant for biosecurity enhancement on and around animal farm.
本研究旨在确定第四代季铵化合物二癸基二甲基溴化铵（DDAB）对家畜行业常见病原体的细菌和病毒去污效果，这些病原体包括婴儿沙门氏菌（SI）、[此处原文缺失部分内容]和禽流感病毒（AIV）。
针对有无有机物质的情况，分别将DDAB配制成百万分之500、250和125（ppm）的溶液。同时，在DDAB溶液样本中加入5%的胎牛血清以模拟有机物质污染的存在。将每种DDAB浓度的400微升与每种病原体（SI、[此处原文缺失部分内容]和AIV）的100微升混合，然后在室温或4°C下于不同时间点（5秒、30秒、1分钟、5分钟、10分钟、15分钟和30分钟）孵育。使用500微升胎牛血清终止DDAB处理的活性。每个处理样本分别在脱氧胆酸盐硫化氢乳糖琼脂平板或Madin-Darby犬肾细胞上进行滴定，用于检测细菌和AIV。每个处理重复进行三次，当减少因子≥3 log时，病原体灭活被认为有效。
我们目前的研究表明，在不同的DDAB浓度、有机物质条件、暴露温度和暴露时间下，DDAB均可灭活SI、[此处原文缺失部分内容]和AIV。此外，杀菌和杀病毒效果的比较表明，与病毒相比，细菌更容易被DDAB灭活。然而，DDAB在有无有机物质的情况下显示出明显的灭活差异。
DDAB可能是一种潜在的消毒剂，可用于家畜养殖场灭活细菌和病毒，尤其是包膜病毒。它可作为一种消毒剂用于增强动物养殖场及其周边的生物安全性。
