Alam Md Shahin, Takahashi Satoru, Ito Mariko, Komura Miyuki, Suzuki Mayuko, Sangsriratanakul Natthanan, Shoham Dany, Takehara Kazuaki
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fchu-shi, Tokyo 183-8509, Japan.
Applied Veterinary Science, United Graduate School of Veterinary Science, Gifu University, 1-1, Yanagido, Gifu 501-1193, Japan.
J Vet Med Sci. 2018 Oct 10;80(10):1482-1489. doi: 10.1292/jvms.18-0390. Epub 2018 Aug 16.
The bactericidal efficacies of 0.2% food additive grade calcium hydroxide (FdCa(OH)) solution, a quaternary ammonium compound (QAC) diluted at 1:500 (QACx500) and their mixture-Mix500 (FdCa(OH) powder added at final concentration 0.2% to QACx500)-were investigated at two different temperatures (room temperature (RT) (25 ± 2°C) and 2°C), using varying contact time, with or without presence of organic materials (5% fetal bovine serum: FBS), either in suspension or on abiotic carrier (steel, rubber and plastic). In the suspension test, QACx500 could inactivate Salmonella Infantis at effective level (≥3 log reductions), within 30 sec and 5 sec, respectively, with or without 5% FBS at RT; however, at 2°C it required 30 min and 1 min, respectively. Mix500 revealed the same efficacy as QACx500 at RT, but, at 2°C it required 1 min and 30 sec, respectively with or without FBS. Whereas, 0.2% FdCa(OH) solution alone could inactivate S. Infantis within 1 min and 3 min, respectively at RT and 2°C, even with 5% FBS. In the carrier test, single disinfectant required bit more (3 or 5 min) contact time to reduce bacterial load (S. Infantis or Escherichia coli) down to the effective level on rubber surface than that on steel and plastic surface. However, Mix500 could inactivate both bacteria on carrier surfaces within 1 min, even at 2°C. Thus, synergistic effects were observed in the suspension test and the carrier test at both temperatures toward both bacteria.
研究了0.2%食品添加剂级氢氧化钙（FdCa(OH)）溶液、以1:500稀释的季铵化合物（QAC）（QACx500）及其混合物Mix500（将FdCa(OH)粉末以最终浓度0.2%添加到QACx500中）在两种不同温度（室温（RT）（25±2°C）和2°C）下的杀菌效果，采用不同的接触时间，有无有机物质（5%胎牛血清：FBS）存在，无论是在悬浮液中还是在非生物载体（钢、橡胶和塑料）上。在悬浮液试验中，QACx500在室温下，无论有无5%FBS，分别在30秒和5秒内可将婴儿沙门氏菌灭活至有效水平（≥3个对数减少）；然而，在2°C时，分别需要30分钟和1分钟。Mix500在室温下显示出与QACx500相同的效果，但在2°C时，无论有无FBS，分别需要1分钟和30秒。而单独的0.2%FdCa(OH)溶液即使在有5%FBS的情况下，在室温下和2°C时分别在1分钟和3分钟内可将婴儿沙门氏菌灭活。在载体试验中，单一消毒剂在橡胶表面将细菌载量（婴儿沙门氏菌或大肠杆菌）降低到有效水平所需的接触时间比在钢和塑料表面稍长（3或5分钟）。然而，Mix500即使在2°C时也能在1分钟内使载体表面的两种细菌灭活。因此，在悬浮液试验和载体试验中均观察到在两种温度下对两种细菌都有协同作用。
