子宫颈癌细胞系中的16型和18型人乳头瘤病毒序列

Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix.

作者信息

Pater M M, Pater A

出版信息

Virology. 1985 Sep;145(2):313-8. doi: 10.1016/0042-6822(85)90164-3.

Abstract

A total of eight human epithelial cell lines derived from the carcinoma of the cervix were examined for the presence of human papillomaviruses (HPVs) types 16 and 18 DNA sequences. Six out of eight cell lines contain sequences hybridizing to the DNA of these viruses. Two of the cell lines contain sequences hybridizing specifically to HPV 16. One of these two cell lines contains all of the HPV 16 sequences and the other cell line is missing fragments containing early regions E2 and E4 and some of the late regions. Four of the cell lines contain sequences hybridizing specifically to HPV 18. All these cell lines are missing fragments containing early regions E2, E4, and E5. Interestingly, all the cell lines contain sequences corresponding to early regions E1, E6, and E7.

摘要

总共对8种源自宫颈癌的人类上皮细胞系进行了检测，以确定是否存在16型和18型人乳头瘤病毒（HPV）的DNA序列。8个细胞系中有6个含有与这些病毒DNA杂交的序列。其中2个细胞系含有与HPV 16特异性杂交的序列。这2个细胞系中的1个含有所有的HPV 16序列，另1个细胞系缺失包含早期区域E2和E4以及一些晚期区域的片段。4个细胞系含有与HPV 18特异性杂交的序列。所有这些细胞系都缺失包含早期区域E2、E4和E5的片段。有趣的是，所有细胞系都含有与早期区域E1、E6和E7相对应的序列。