Detection of leukotoxin and fimbria-associated protein gene genotypes among periodontitis patients and healthy controls: A case-control study.

BACKGROUND

has been reported in higher proportions in subgingival microbiota of individuals with aggressive periodontitis (AgP) compared with those with chronic periodontitis (ChP) and healthy controls. The major virulence factors are the ones that help in colonization and evasion of host's defenses. Hence, this study was aimed to assess the prevalence of and its virulent genotypes (leukotoxin [] and fimbria-associated protein []).

MATERIALS AND METHODS

In this case- control study We performed periodontal examination and measured probing depth and clinical attachment level (CAL). Subgingival plaque samples from 200 (ChP: = 128 and AgP: = 72) periodontitis patients and 200 healthy controls were screened for the presence of , , and genotypes by polymerase chain reaction. The prevalence of genotypes between periodontitis patients and healthy controls was compared with Pearson's Chi-square test. < 0.05 was considered statistically significant.

RESULTS

Mean pocket probing depth and CAL were high as compared to the healthy controls. The prevalence of in ChP ( = 128), AgP ( = 72), and healthy individuals ( = 200) was 32.0%, 61.1%, and 2.5%, respectively. genotype prevalence was 71.8% among periodontitis patients, while genotype showed 31.8% prevalence. The prevalence of these genotypes was insignificant in healthy controls.

CONCLUSION

The high odds ratio for prevalence suggests its strong link to periodontitis. Detection of + genotype may be a useful marker for AgP as its prevalence was found to be high in AgP.