Mahalakshmi Krishnan, Krishnan Padma, Chandrasekaran S C
Department of Microbiology and Research Lab for Oral-Systemic Health, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
Department of Microbiology, Dr. ALM PGIBMS, University of Madras, Chennai, Tamil Nadu, India.
Dent Res J (Isfahan). 2018 May-Jun;15(3):185-190.
has been reported in higher proportions in subgingival microbiota of individuals with aggressive periodontitis (AgP) compared with those with chronic periodontitis (ChP) and healthy controls. The major virulence factors are the ones that help in colonization and evasion of host's defenses. Hence, this study was aimed to assess the prevalence of and its virulent genotypes (leukotoxin [] and fimbria-associated protein []).
In this case- control study We performed periodontal examination and measured probing depth and clinical attachment level (CAL). Subgingival plaque samples from 200 (ChP: = 128 and AgP: = 72) periodontitis patients and 200 healthy controls were screened for the presence of , , and genotypes by polymerase chain reaction. The prevalence of genotypes between periodontitis patients and healthy controls was compared with Pearson's Chi-square test. < 0.05 was considered statistically significant.
Mean pocket probing depth and CAL were high as compared to the healthy controls. The prevalence of in ChP ( = 128), AgP ( = 72), and healthy individuals ( = 200) was 32.0%, 61.1%, and 2.5%, respectively. genotype prevalence was 71.8% among periodontitis patients, while genotype showed 31.8% prevalence. The prevalence of these genotypes was insignificant in healthy controls.
The high odds ratio for prevalence suggests its strong link to periodontitis. Detection of + genotype may be a useful marker for AgP as its prevalence was found to be high in AgP.
与慢性牙周炎（ChP）患者和健康对照相比，侵袭性牙周炎（AgP）患者龈下微生物群中[某种物质]的比例更高。主要毒力因子是那些有助于定植和逃避宿主防御的因子。因此，本研究旨在评估[某种物质]及其毒力基因型（白细胞毒素[具体名称]和菌毛相关蛋白[具体名称]）的患病率。
在本病例对照研究中，我们进行了牙周检查并测量了探诊深度和临床附着水平（CAL）。通过聚合酶链反应对200例牙周炎患者（ChP：n = 128，AgP：n = 72）和200例健康对照的龈下菌斑样本进行筛选，以检测[某种物质]、[具体物质1]和[具体物质2]基因型的存在情况。采用Pearson卡方检验比较牙周炎患者和健康对照之间基因型的患病率。P < 0.05被认为具有统计学意义。
与健康对照相比，平均牙周袋探诊深度和CAL较高。ChP组（n = 128）、AgP组（n = 72）和健康个体组（n = 200）中[某种物质]的患病率分别为32.0%、61.1%和2.5%。牙周炎患者中[具体物质1]基因型患病率为71.8%，而[具体物质2]基因型患病率为31.8%。这些基因型在健康对照中的患病率无统计学意义。
[某种物质]患病率的高比值比表明其与牙周炎有很强的关联。检测[具体物质1] +基因型可能是AgP的一个有用标志物，因为在AgP中发现其患病率很高。
