一株对唑类药物有耐药性的厚皮马拉色菌分离株。
An azole-resistant isolate of Malassezia pachydermatis.
机构信息
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
出版信息
Vet Microbiol. 2011 Apr 21;149(1-2):288-90. doi: 10.1016/j.vetmic.2010.10.010. Epub 2010 Oct 21.
Canine Malassezia dermatitis (MD) is frequently treated with systemic ketoconazole (KTZ) and itaconazole (ITZ). However, the antifungal susceptibility of clinical isolates of M. pachydermatis from dogs and cats to the azoles has not been well investigated. In the present study, the in vitro susceptibility of the standard strain (CBS1879: the neotype strain of M. pachydermatis) and 29 clinical isolates of M. pachydermatis to the azoles was measured by a modified CLSI M27-A2 test using modified Dixon medium as well as by the E-test. The minimum inhibitory concentrations (MICs) of the 30 isolates of M. pachydermatis (including the neotype strain) against KTZ and ITZ were <0.03 μg/ml by the two methods. The MICs of 1 clinical isolate (ASC-11) were 1 and 2 μg/ml against KTZ, and 2 and 8 μg/ml against ITZ, by the modified CLSI M27-A2 test and the E-test, respectively. Thus, isolate ASC-11 may be resistant to these azoles, making this the first report of a resistant isolate of M. pachydermatis to KTZ and ITZ.
犬马拉色菌性皮炎（MD）常采用系统性酮康唑（KTZ）和伊曲康唑（ITZ）进行治疗。然而，犬猫源厚皮马拉色菌临床分离株对唑类药物的抗真菌敏感性尚未得到充分研究。在本研究中，采用改良的 CLSI M27-A2 试验，使用改良的 Dixon 培养基和 E 试验，测定了标准株（CBS1879：厚皮马拉色菌的新种株）和 29 株临床分离株对唑类药物的体外敏感性。30 株厚皮马拉色菌（包括新种株）对 KTZ 和 ITZ 的最小抑菌浓度（MICs）均<0.03μg/ml，两种方法均如此。临床分离株 ASC-11 对 KTZ 的 MIC 分别为 1 和 2μg/ml，对 ITZ 的 MIC 分别为 2 和 8μg/ml，这是通过改良的 CLSI M27-A2 试验和 E 试验分别测定的。因此，分离株 ASC-11 可能对这些唑类药物具有耐药性，这是首例报道的对 KTZ 和 ITZ 耐药的厚皮马拉色菌分离株。
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