Department of Family Medicine, University of Ulsan College of Medicine, 88, Olympic-Ro 43-Gil, Songpa-gu, Seoul, 138-736, South Korea.
Obes Surg. 2013 Apr;23(4):554-60. doi: 10.1007/s11695-012-0801-2.
Limited data are available on the in vivo expression of fibronectin, one of the main extracellular matrix components. We investigated the expression of fibronectin in abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) and the associations of leptin, adiponectin, and vaspin gene expression with metabolic parameters in obese women.
We recruited female subjects undergoing bariatric surgery for obesity (n = 24) and patients undergoing benign gynecological surgery as the control group (n = 23). We measured anthropometric variables, abdominal fat distribution, metabolic parameters, serum concentrations of leptin, adiponectin, and vaspin, and depot-specific mRNA expression of fibronectin, leptin, adiponectin, and vaspin.
Fibronectin expression in both VAT and SAT was significantly lower in the obese group than in the control group. Fibronectin expression in both VAT and SAT were negatively correlated with body mass index or waist circumference, with higher prominence in VAT. In multiple regression analysis, fibronectin expression in both VAT and SAT was negatively correlated with serum leptin concentration. Fibronectin expression in VAT was negatively correlated with leptin expression in VAT. Additionally, fibronectin expression in SAT was negatively correlated with leptin expression in SAT and positively correlated with adiponectin expression in VAT and SAT.
We found significant negative associations between depot-specific fibronectin expression in human adipose tissue and obesity indices and obesity-related biomarkers. Our results suggest that fibronectin expression may contribute to obesity and metabolic dysregulation in humans.
关于细胞外基质主要成分之一纤维连接蛋白的体内表达，目前仅有有限的数据。我们研究了肥胖女性腹部内脏脂肪组织（VAT）和皮下脂肪组织（SAT）中纤维连接蛋白的表达情况，以及瘦素、脂联素和vaspin 基因表达与代谢参数的相关性。
我们招募了因肥胖而行减重手术的女性患者（n=24）和因良性妇科手术而行手术的患者作为对照组（n=23）。我们测量了人体测量学变量、腹部脂肪分布、代谢参数、血清瘦素、脂联素和 vaspin 浓度以及脂肪组织中纤维连接蛋白、瘦素、脂联素和 vaspin 的基因表达。
肥胖组的 VAT 和 SAT 中的纤维连接蛋白表达明显低于对照组。VAT 和 SAT 中的纤维连接蛋白表达与体重指数或腰围呈负相关，VAT 中的相关性更为显著。在多元回归分析中，VAT 和 SAT 中的纤维连接蛋白表达与血清瘦素浓度呈负相关。VAT 中的纤维连接蛋白表达与 VAT 中的瘦素表达呈负相关。此外，SAT 中的纤维连接蛋白表达与 SAT 中的瘦素表达呈负相关，与 VAT 和 SAT 中的脂联素表达呈正相关。
我们发现人类脂肪组织中特定部位的纤维连接蛋白表达与肥胖指数和肥胖相关生物标志物之间存在显著的负相关。我们的结果表明，纤维连接蛋白表达可能导致肥胖和人类代谢失调。
