钙参与咖啡因对人类精子活力的刺激作用。
Involvement of calcium in the caffeine stimulation of human sperm motility.
作者信息
Hong C Y, Chiang B N, Wu P, Wei Y H, Fong J C
出版信息
Br J Clin Pharmacol. 1985 Jun;19(6):739-43. doi: 10.1111/j.1365-2125.1985.tb02708.x.
The shape of the concentration-response curve and the amplitude of maximal motility increase for the stimulatory effect of caffeine on human sperm motility were similar to those of EGTA, a calcium chelator, and lanthanum chloride, a calcium entry blocker. On the other hand, the maximal motility stimulation induced by dibutyryl cyclic AMP was similar to that induced by trifluoperazine, a calmodulin antagonist. The antagonism of A23187 on the motility-stimulating effect of EGTA and caffeine was competitive in nature, while that on cyclic AMP was non-competitive. We proposed that there could be two mechanisms for caffeine to stimulate sperm motility: at higher concentrations, caffeine modified calcium translocation; at lower concentrations, caffeine increased cyclic AMP level. The importance of calcium in the regulation of human sperm motility was reconfirmed in this study.
咖啡因对人类精子活力的刺激作用所产生的浓度-反应曲线形状以及最大活力增加幅度，与钙螯合剂乙二醇双四乙酸（EGTA）和钙内流阻滞剂氯化镧相似。另一方面，二丁酰环磷酸腺苷（dibutyryl cyclic AMP）诱导的最大活力刺激与钙调蛋白拮抗剂三氟拉嗪诱导的相似。A23187对EGTA和咖啡因活力刺激作用的拮抗本质上是竞争性的，而对环磷酸腺苷（cyclic AMP）的拮抗是非竞争性的。我们提出，咖啡因刺激精子活力可能有两种机制：在较高浓度下，咖啡因改变钙转运；在较低浓度下，咖啡因增加环磷酸腺苷水平。本研究再次证实了钙在调节人类精子活力中的重要性。
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