Dalmastri Claudia, Han Weihua, Vespucci Stefano, Wang Liqian, Morales Piero
ENEA, Centro Ricerche Casaccia, Rome, Italy.
Centro NAST, Università di Tor Vergata, Rome, Italy.
Methods Mol Biol. 2018;1811:263-278. doi: 10.1007/978-1-4939-8582-1_18.
We describe here one way to achieve hybrid DNA-inorganic nanostructures on rigid flat insulating substrates. We report methods to prepare rectangular DNA origami and incubate them onto arrays of anchoring gold nanodots either in a static solution or in a microfluidic system. We give details on the design and lithographic methods employed to pattern usable arrays of gold nanoanchors on naturally oxidized silicon wafer chips. Scanning electron and atomic force microscopy methodological details are also given for obtaining the relevant characterizations of the immobilized and ordered DNA origami.
我们在此描述一种在刚性平面绝缘基板上实现混合DNA-无机纳米结构的方法。我们报告了制备矩形DNA折纸并将其在静态溶液或微流控系统中孵育到锚定金纳米点阵列上的方法。我们详细介绍了用于在天然氧化硅晶片芯片上对可用的金纳米锚阵列进行图案化的设计和光刻方法。还给出了扫描电子显微镜和原子力显微镜的方法细节，以获得固定化且有序的DNA折纸的相关表征。
