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RNA去甲基化酶FTO过表达对硅藻生物量和生物活性物质的影响

Effect of RNA Demethylase FTO Overexpression on Biomass and Bioactive Substances in Diatom .

作者信息

Yang Yanan, Yang Min, Zhou Yihang, Chen Xiaoqian, Huang Bingyao

机构信息

Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China.

University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Nanning 530200, China.

出版信息

Biology (Basel). 2025 Apr 13;14(4):414. doi: 10.3390/biology14040414.

DOI:10.3390/biology14040414
PMID:40282280
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12024952/
Abstract

is rich in bioactive substances, rendering it valuable in nutrition and medicine. Epigenetic editing mediated by human RNA demethylase FTO can significantly increase the yields of rice and potato and offers significant potential for the genetic breeding of microalgae. This study aimed to enhance the production of certain metabolites in via FTO-mediated epigenetic editing. Phenotypic analysis revealed that transgenic exhibits significantly reduced RNA mA modification levels and faster growth, producing markedly higher levels of lipids, proteins, and carotenoids than the wild type. Transcriptome analysis revealed 1009 upregulated genes and 378 downregulated genes. KEGG analysis demonstrated the upregulated expression of multiple key enzymes involved in long-chain fatty acid synthesis (e.g., ACSL, fabF, and fabG), carotenoid synthesis (e.g., crtQ, PDS, and PSY1), and amino acid synthesis (e.g., dapF, glyA, and aroK) in transgenic , consistent with our phenotypic results. These results indicate that FTO can promote growth and increase the bioactive compound content in by regulating the mA modification of RNA, and further suggest that FTO has the potential to serve as a new tool for the epigenetic editing of microalgae.

摘要

富含生物活性物质，使其在营养和医学方面具有价值。由人类RNA去甲基化酶FTO介导的表观遗传编辑可显著提高水稻和马铃薯的产量，并为微藻的遗传育种提供巨大潜力。本研究旨在通过FTO介导的表观遗传编辑提高某种微藻中特定代谢物的产量。表型分析表明，转基因微藻的RNA mA修饰水平显著降低且生长更快，产生的脂质、蛋白质和类胡萝卜素水平明显高于野生型。转录组分析揭示了1009个上调基因和378个下调基因。KEGG分析表明，转基因微藻中参与长链脂肪酸合成（如ACSL、fabF和fabG）、类胡萝卜素合成（如crtQ、PDS和PSY1）和氨基酸合成（如dapF、glyA和aroK）的多种关键酶的表达上调，与我们的表型结果一致。这些结果表明，FTO可通过调节RNA的mA修饰促进微藻生长并增加生物活性化合物含量，进一步表明FTO有潜力作为微藻表观遗传编辑的新工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/12024952/280f1dca2cda/biology-14-00414-g007.jpg
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本文引用的文献

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Comparative Transcriptomics to Identify RNA Writers and Erasers in Microalgae.比较转录组学鉴定微藻中的 RNA 写入器和擦除器。
Int J Mol Sci. 2024 Jul 23;25(15):8005. doi: 10.3390/ijms25158005.
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The model diatom Phaeodactylum tricornutum provides insights into the diversity and function of microeukaryotic DNA methyltransferases.模式硅藻三角褐指藻为研究真核生物 DNA 甲基转移酶的多样性和功能提供了线索。
Commun Biol. 2023 Mar 9;6(1):253. doi: 10.1038/s42003-023-04629-0.
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CRISPR technology: A decade of genome editing is only the beginning.
CRISPR技术：基因组编辑的十年仅仅是个开始。
Science. 2023 Jan 20;379(6629):eadd8643. doi: 10.1126/science.add8643.
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Common Pathogenetic Mechanisms Underlying Aging and Tumor and Means of Interventions.衰老与肿瘤的共同发病机制及干预手段。
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RNA demethylation increases the yield and biomass of rice and potato plants in field trials.RNA 去甲基化可提高田间试验中水稻和马铃薯植株的产量和生物量。
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CRISPR-Cas and Its Wide-Ranging Applications: From Human Genome Editing to Environmental Implications, Technical Limitations, Hazards and Bioethical Issues.CRISPR-Cas 及其广泛的应用：从人类基因组编辑到环境影响、技术限制、危害和生物伦理问题。
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J Int Med Res. 2020 Oct;48(10):300060520964011. doi: 10.1177/0300060520964011.
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