Melioli G, Pedullà D, Merli A L, Arata L, Leonardi A
Diagn Microbiol Infect Dis. 1985 Sep;3(5):411-7. doi: 10.1016/0732-8893(85)90080-x.
The intrathecal production of antimeasles antibodies was studied using the enzyme-linked immunosorbent assay in eight specimens of serum and cerebrospinal fluid (CSF) from patients with clinical signs of subacute sclerosing panencephalitis (SSPE). The test was performed using a 1:5 dilution of CSF and a 1:2000 dilution of serum (ratio 1:400) in order to nullify the physiological gradient of immunoglobulins across the blood brain barrier (BBB). This procedure allowed a rapid and accurate assessment of the synthesis of specific immunoglobulins in the CSF and a good evaluation of the permeability of the BBB. A diagnosis of SSPE was provided in five out of eight patients with clinical signs of the disease. Clinical follow-up confirmed the diagnosis of SSPE in the group of patients with clear evidence of intrathecal synthesis of antimeasles antibodies.
采用酶联免疫吸附测定法，对8例有亚急性硬化性全脑炎（SSPE）临床症状患者的血清和脑脊液（CSF）样本进行研究，以检测鞘内抗麻疹抗体的产生情况。检测时，将脑脊液稀释1:5，血清稀释1:2000（比例为1:400），以消除免疫球蛋白通过血脑屏障（BBB）的生理梯度。该方法能够快速、准确地评估脑脊液中特异性免疫球蛋白的合成情况，并很好地评估血脑屏障的通透性。8例有该疾病临床症状的患者中，5例被诊断为SSPE。临床随访证实，在有明确证据表明鞘内合成抗麻疹抗体的患者组中，诊断为SSPE。
