Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia.
Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia; Grupo Investigación Ciencias Forenses y de la Salud, Facultad de Derecho y Ciencias Forenses, Institución Universitaria-Tecnológico de Antioquia, Medellín, Colombia.
Infect Genet Evol. 2018 Oct;64:76-84. doi: 10.1016/j.meegid.2018.06.018. Epub 2018 Jun 18.
The Arribalzagia Series of the Anopheles Subgenus comprises morphologically similar species or members of species complexes which makes correct species identification difficult. Therefore, the aim of this work was to discriminate the morphospecies of the Arribalzagia Series present in Colombia using a multilocus approach based on ITS2, COI and CAD sequences. Specimens of the Arribalzagia Series collected at 32 localities in nine departments were allocated to seven species. Individual and concatenated Bayesian analyses showed high support for each of the species and reinforced the previous report of the Apicimacula species Complex with distribution in the Pacific Coast and northwestern Colombia. In addition, a new molecular operational taxonomic unit-MOTU was identified, herein denominated near Anopheles peryassui, providing support for the existence of a Peryassui species Complex. Further, the CAD gene, just recently used for Anopheles taxonomy and phylogeny, demonstrated its power in resolving phylogenetic relationships among species of the Arribalzagia Series. The divergence times for these species correspond to the early Pliocene and the Miocene. Considering the epidemiological importance of some species of the Series and their co-occurrence in malaria endemic regions of Colombia, their discrimination constitutes an important step for vector incrimination and control in the country.
阿利巴拉扎加亚系列属于按蚊亚属，包含形态相似的种或种复合体，这使得正确的物种鉴定变得困难。因此，本工作旨在利用基于 ITS2、COI 和 CAD 序列的多位点方法来区分哥伦比亚存在的阿利巴拉扎加亚系列的形态种。在 9 个省的 32 个地点收集的阿利巴拉扎加亚系列标本被分配到 7 个种。个体和连锁贝叶斯分析为每个种提供了高度支持，并加强了以前关于分布在太平洋沿岸和哥伦比亚西北部的 Apicimacula 种复合体的报告。此外，还确定了一个新的分子分类操作单元（MOTU），在此命名为近似按蚊 peryassui，为存在 peryassui 种复合体提供了支持。此外，CAD 基因最近被用于按蚊分类学和系统发育，它证明了在解决阿利巴拉扎加亚系列种间系统发育关系方面的强大功能。这些种的分歧时间对应于上新世早期和中新世。考虑到该系列的一些种具有流行病学重要性，并且它们在哥伦比亚疟疾流行地区同时出现，对它们进行区分是在该国进行媒介定罪和控制的重要步骤。
