在PCC4胚胎癌细胞上筛选出的多瘤病毒突变体的共同特征。
Common features of polyomavirus mutants selected on PCC4 embryonal carcinoma cells.
作者信息
Melin F, Pinon H, Reiss C, Kress C, Montreau N, Blangy D
出版信息
EMBO J. 1985 Jul;4(7):1799-803. doi: 10.1002/j.1460-2075.1985.tb03853.x.
Abstract
The genomic rearrangements of six polyomavirus mutants selected on PCC4 embryonal carcinoma cells have been compared and their common characteristics pointed out. All mutants show a duplication which includes at least the adenovirus type 5 (Ad5) E1A-like enhancer core sequence plus a deletion of variable size and location. The presence of the second enhancer core sequence, the SV40-like enhancer, is not required for expression of the PyEC PCC4 phenotype. Two of these mutants are also able to express polyomavirus T antigen on F9 and LT1 cells. Multiadaptation seems to require the duplication of the Ad5 E1A-like core sequence, the maintenance of the SV40-like core sequence and a local change in DNA stability.
摘要
已对在PCC4胚胎癌细胞上筛选出的六种多瘤病毒突变体的基因组重排进行了比较，并指出了它们的共同特征。所有突变体均显示出重复，该重复至少包括5型腺病毒（Ad5）E1A样增强子核心序列，外加大小和位置可变的缺失。PyEC PCC4表型的表达不需要第二个增强子核心序列，即SV40样增强子的存在。这些突变体中的两个也能够在F9和LT1细胞上表达多瘤病毒T抗原。多重适应性似乎需要Ad5 E1A样核心序列的重复、SV40样核心序列的维持以及DNA稳定性的局部变化。
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本文引用的文献
1
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Biochimie. 1982 Feb;64(2):99-112. doi: 10.1016/s0300-9084(82)80412-4.
2
The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element.5型腺病毒E1A转录控制区包含一个重复的增强子元件。
Cell. 1983 Jul;33(3):695-703. doi: 10.1016/0092-8674(83)90012-0.
3
Isolation and characterization of polyoma virus mutants which grow in murine embryonal carcinoma and trophoblast cells.在小鼠胚胎癌细胞和滋养层细胞中生长的多瘤病毒突变体的分离与鉴定。
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4
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Cell. 1983 Dec;35(3 Pt 2):693-9. doi: 10.1016/0092-8674(83)90102-2.
5
Comparison of the DNA sequence of the Crawford small-plaque variant of polyomavirus with those of polyomaviruses A2 and strain 3.多瘤病毒克劳福德小斑块变异株与多瘤病毒A2及3型毒株的DNA序列比较。
J Virol. 1983 Nov;48(2):472-80. doi: 10.1128/JVI.48.2.472-480.1983.
6
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7
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Science. 1983 Feb 11;219(4585):626-31. doi: 10.1126/science.6297005.
9
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J Mol Biol. 1982 Nov 15;161(4):533-50. doi: 10.1016/0022-2836(82)90406-5.
10
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