包虫囊肿壁的一种53千道尔顿糖抗原可能参与逃避宿主免疫系统。
A 53 KDa Glycan Antigen of Hydatid Cyst Wall May Involve in Evasion from Host Immune System.
作者信息
Sefiddashti Raheleh Rafiei, Sharafi Seyedeh Maryam, Ebrahimi Soltan Ahmad, Akhlaghi Lame, Moosavi Ali, Eskandarian Abasali, Darani Hossein Yousofi
机构信息
Department of Medical Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Infectious Diseases and Tropical Medicine Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Adv Biomed Res. 2018 May 23;7:82. doi: 10.4103/abr.abr_287_16. eCollection 2018.
BACKGROUND
Recent studies have shown that similar host glycan antigens are expressed by helminths such as hydatid cysts to evade from host immune system. In this work to investigate these antigens further, immunological cross-reactivity between human sera and hydatid cyst wall antigens has been investigated.
MATERIALS AND METHODS
Hydatid cyst wall antigens were used in enzyme-linked immunosorbent assay and Western immunoblotting and probed with pooled sera of hydatidosis patients and healthy controls. Sodium metaperiodate treatment was used to investigate glycan antigens.
RESULTS
A band with molecular weight about 53 KDa reacted with both hydatid patients' sera and also normal human sera. It has been shown that this band was a glycan antigen.
CONCLUSIONS
A 53 KDa glycan antigen of hydatid cyst wall that reacted with all human sera may have an important role for evasion from host immune system.
背景
最近的研究表明，诸如包虫囊肿等蠕虫表达相似的宿主聚糖抗原以逃避宿主免疫系统。在这项进一步研究这些抗原的工作中，对人血清与包虫囊肿壁抗原之间的免疫交叉反应性进行了研究。
材料与方法
将包虫囊肿壁抗原用于酶联免疫吸附测定和 Western 免疫印迹，并用人包虫病患者和健康对照的混合血清进行检测。使用偏高碘酸钠处理来研究聚糖抗原。
结果
一条分子量约为 53 kDa 的条带与人包虫病患者血清和正常人血清均发生反应。已表明该条带是一种聚糖抗原。
结论
包虫囊肿壁的一种 53 kDa 聚糖抗原与所有人血清均发生反应，可能在逃避宿主免疫系统方面发挥重要作用。
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