Hebert C N, Edwards S, Bushnell S, Jones P C, Perry C T
J Biol Stand. 1985 Jul;13(3):243-53. doi: 10.1016/s0092-1157(85)80008-1.
The critical statistical parameters of an enzyme-linked immunosorbent assay were determined to enable quantitation of antibody responses in cattle affected with infectious bovine rhinotracheitis. A system of controlling well-to-well variations in optical density reading across a microtitre plate was evolved and dose--response assays were carried out to determine the dilution of serum which gave the greatest discrimination between acute and convalescent sera from an infected animal. Use of a standard serum was studied in further assays. An increase in optical density value of 0.15 was set as a diagnostic criterion for a significantly rising antibody response. This compared well with the conventional criterion of a fourfold rise in virus neutralizing antibody titre.
测定了酶联免疫吸附测定的关键统计参数，以定量感染牛传染性鼻气管炎的牛的抗体反应。建立了一个控制系统，用于控制微量滴定板上各孔光密度读数的差异，并进行剂量反应测定，以确定能最大程度区分感染动物急性血清和恢复期血清的血清稀释度。在进一步的测定中研究了标准血清的使用。将光密度值增加0.15设定为抗体反应显著升高的诊断标准。这与病毒中和抗体滴度升高四倍的传统标准相当。
