Perrin B, Calvo T, Cordioli P, Coudert M, Edwards S, Eloit M, Guérin B, Kramps J A, Lenihan P, Paschaleri E
Centre National d'Etudes Vétérinaires et Alimentaires-Laboratoire de Pathologie Bovine, Lyons, France.
Rev Sci Tech. 1994 Sep;13(3):947-60. doi: 10.20506/rst.13.3.810.
A study, consisting of three trials, was undertaken in order to find standard reference sera for use in serological diagnostic procedures for infectious bovine rhinotracheitis (IBR) in the European Union (EU). A total of forty-nine laboratories participated in the trials, in which panels of positive, weak positive, and negative candidate sera were tested blind by neutralisation, indirect and blocking enzyme-linked immunosorbent assay, indirect immunofluorescence and passive haemagglutination. A serum which scored positive in all tests in all laboratories was selected as a strong positive standard serum. The chosen negative standard serum gave negative results in virtually all tests. A 1/36 dilution of a positive serum was chosen as the weak positive standard serum and only very sensitive techniques were able to score it positive. Three EU reference sera are now available for the standardisation of serological tests for IBR.
为了找到用于欧盟（EU）牛传染性鼻气管炎（IBR）血清学诊断程序的标准参考血清，开展了一项由三项试验组成的研究。共有49个实验室参与了这些试验，其中通过中和试验、间接和阻断酶联免疫吸附测定、间接免疫荧光和被动血凝试验对阳性、弱阳性和阴性候选血清组进行了盲法检测。在所有实验室的所有检测中均呈阳性的一种血清被选为强阳性标准血清。所选的阴性标准血清在几乎所有检测中均给出阴性结果。一种阳性血清的1/36稀释液被选为弱阳性标准血清，只有非常灵敏的技术才能将其判定为阳性。现在有三种欧盟参考血清可用于IBR血清学检测的标准化。
