对导致肺炎的多药耐药菌希瓦氏菌的抗药基因组进行测序解析，法国马赛。
Whole genome sequence to decipher the resistome of Shewanella algae, a multidrug-resistant bacterium responsible for pneumonia, Marseille, France.
机构信息
a URMITE UM 63 CNRS 7278 IRD 198 INSERM U1905, Méditerranée Infection, Facultés de Médecine et de Pharmacie , Aix-Marseille Université , Marseille Cedex 05 , France.
b Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie , Centre Hospitalo-Universitaire Timone, Méditerranée Infection , Marseille Cedex 05 , France.
出版信息
Expert Rev Anti Infect Ther. 2016;14(2):269-75. doi: 10.1586/14787210.2016.1106936. Epub 2015 Nov 2.
UNLABELLED
We characterize and decipher the resistome and the virulence factors of Shewanella algae MARS 14, a multidrug-resistant clinical strain using the whole genome sequencing (WGS) strategy. The bacteria were isolated from the bronchoalveolar lavage of a hospitalized patient in the Timone Hospital in Marseille, France who developed pneumonia after plunging into the Mediterranean Sea.
RESULTS
The genome size of S. algae MARS 14 was 5,005,710 bp with 52.8% guanine cytosine content. The resistome includes members of class C and D beta-lactamases and numerous multidrug-efflux pumps. We also found the presence of several hemolysins genes, a complete flagellum system gene cluster and genes responsible for biofilm formation. Moreover, we reported for the first time in a clinical strain of Shewanella spp. the presence of a bacteriocin (marinocin).
CONCLUSION
The WGS analysis of this pathogen provides insight into its virulence factors and resistance to antibiotics.
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我们使用全基因组测序（WGS）策略对多药耐药临床株希瓦氏菌 MARS 14 的耐药组和毒力因子进行了表征和解析。该细菌是从法国马赛蒂姆诺医院一位住院患者的支气管肺泡灌洗液中分离出来的，该患者在潜入地中海后患上了肺炎。
结果
希瓦氏菌 MARS 14 的基因组大小为 5005710bp，鸟嘌呤-胞嘧啶含量为 52.8%。耐药组包括 C 类和 D 类β-内酰胺酶以及许多多药外排泵。我们还发现了几种溶血素基因、一个完整的鞭毛系统基因簇和负责生物膜形成的基因。此外，我们首次在希瓦氏菌的临床株中报道了细菌素（海菌素）的存在。
结论
对该病原体的 WGS 分析深入了解了其毒力因子和对抗生素的耐药性。
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