用于1型和3型轮状病毒的特异性酶联免疫测定法。
Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.
作者信息
Shaw R D, Stoner-Ma D L, Estes M K, Greenberg H B
出版信息
J Clin Microbiol. 1985 Aug;22(2):286-91. doi: 10.1128/jcm.22.2.286-291.1985.
Abstract
We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies which react specifically with VP7 of each serotype were identified by hemagglutination inhibition tests, plaque reduction neutralization studies, and solid-phase immunoassays which used wild-type and reassortant strains of rotavirus. An enzyme-linked immunoassay was designed which utilizes two of these antibodies to correctly identify serotype 1 and serotype 3 viruses.
摘要
我们制备了针对两种血清型不同的轮状病毒株的单克隆抗体
Wa，一种源自人类的血清型1病毒，以及恒河猴轮状病毒，一种猿猴血清型3病毒。通过血凝抑制试验、蚀斑减少中和研究以及使用轮状病毒野生型和重配株的固相免疫测定，鉴定出了与每种血清型的VP7特异性反应的单克隆抗体。设计了一种酶联免疫测定法，该方法利用其中两种抗体来正确鉴定血清型1和血清型3病毒。
相似文献
1
Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.用于1型和3型轮状病毒的特异性酶联免疫测定法。
J Clin Microbiol. 1985 Aug;22(2):286-91. doi: 10.1128/jcm.22.2.286-291.1985.
2
Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7.通过使用针对VP7的1型、2型、3型和4型特异性单克隆抗体的酶联免疫吸附测定法对粪便中的人轮状病毒进行直接血清分型。
J Infect Dis. 1987 Jun;155(6):1159-66. doi: 10.1093/infdis/155.6.1159.
3
Identification of the two rotavirus genes determining neutralization specificities.确定决定中和特异性的两种轮状病毒基因。
J Virol. 1986 Jan;57(1):376-8. doi: 10.1128/JVI.57.1.376-378.1986.
4
Development of a monoclonal antibody specific for serotype 3 rotavirus strains.针对3型轮状病毒株的单克隆抗体的研制
Eur J Clin Microbiol. 1987 Apr;6(2):136-41. doi: 10.1007/BF02018194.
5
Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.使用单克隆抗体对人轮状病毒P1A和P2血清型进行血清学分析。
J Clin Microbiol. 1993 Mar;31(3):622-8. doi: 10.1128/jcm.31.3.622-628.1993.
6
Determination of rotavirus serotype-specific antibodies in sera by competitive enhanced enzyme immunoassay.
J Virol Methods. 1989 Apr-May;24(1-2):103-10. doi: 10.1016/0166-0934(89)90012-8.
7
Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens.一种使用血清型特异性单克隆抗体的酶联免疫吸附测定法对粪便标本中人类轮状病毒进行血清分型的有效性。
Microbiol Immunol. 1988;32(7):699-708. doi: 10.1111/j.1348-0421.1988.tb01431.x.
8
Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3.人轮状病毒中和性单克隆抗体的衍生及血清型1和3之间存在共同免疫显性中和位点的证据
Virology. 1986 Oct 30;154(2):302-12. doi: 10.1016/0042-6822(86)90456-3.
9
Cross-reactive, serotype- and monotype-specific neutralization epitopes on VP7 of serotype G3 and G5 porcine rotavirus strains.G3和G5型猪轮状病毒株VP7上的交叉反应性、血清型和单型特异性中和表位
Arch Virol. 1996;141(3-4):601-14. doi: 10.1007/BF01718320.
10
Neutralizing monoclonal antibodies against three serotypes of porcine rotavirus.针对三种血清型猪轮状病毒的中和单克隆抗体
J Virol. 1989 Aug;63(8):3545-9. doi: 10.1128/JVI.63.8.3545-3549.1989.
引用本文的文献
1
VP4- and VP7-specific antibodies mediate heterotypic immunity to rotavirus in humans.针对VP4和VP7的抗体介导人类对轮状病毒的异型免疫。
Sci Transl Med. 2017 Jun 21;9(395). doi: 10.1126/scitranslmed.aam5434.
2
The shift from low to high non-structural protein 1 expression in rotavirus-infected MA-104 cells.轮状病毒感染的 MA-104 细胞中非结构蛋白 1 从低到高的表达变化。
Mem Inst Oswaldo Cruz. 2013 Jun;108(4):421-8. doi: 10.1590/S0074-0276108042013005.
3
Intestinal epithelia activate anti-viral signaling via intracellular sensing of rotavirus structural components.肠上皮细胞通过细胞内感应轮状病毒结构成分来激活抗病毒信号。
Mucosal Immunol. 2010 Nov;3(6):622-32. doi: 10.1038/mi.2010.39. Epub 2010 Jul 21.
4
Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes.用于A组轮状病毒VP4（P[4]、P[6]、P[8]、P[9]和P[14]）及VP7（G1至G6、G8至G10和G12）基因基因分型的DNA微阵列的开发与验证
J Clin Microbiol. 2007 Aug;45(8):2641-8. doi: 10.1128/JCM.00736-07. Epub 2007 Jun 13.
5
Rotavirus serotype G9 strains belonging to VP7 gene phylogenetic sequence lineage 1 may be more suitable for serotype G9 vaccine candidates than those belonging to lineage 2 or 3.与属于第2或3分支的轮状病毒G9毒株相比，属于VP7基因系统发育序列第1分支的轮状病毒G9毒株可能更适合作为G9血清型候选疫苗。
J Virol. 2004 Jul;78(14):7795-802. doi: 10.1128/JVI.78.14.7795-7802.2004.
6
Rotavirus typing methods and algorithms.轮状病毒分型方法及算法。
Rev Med Virol. 2004 Mar-Apr;14(2):71-82. doi: 10.1002/rmv.411.
7
Antigenic and genetic characterization of serotype G2 human rotavirus strains from the African continent.来自非洲大陆的G2型人类轮状病毒株的抗原性和基因特征分析。
J Clin Microbiol. 2004 Feb;42(2):595-600. doi: 10.1128/JCM.42.2.595-600.2004.
8
Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype.与血清分型失败相关的G2轮状病毒株VP7蛋白内的氨基酸替换。
J Clin Microbiol. 2001 Oct;39(10):3796-8. doi: 10.1128/JCM.39.10.3796-3798.2001.
9
Distribution of rotavirus VP7 serotypes and VP4 genotypes circulating in Sousse, Tunisia, from 1995 to 1999: emergence of natural human reassortants.1995年至1999年突尼斯苏塞市流行的轮状病毒VP7血清型和VP4基因型分布：天然人重组体的出现
J Clin Microbiol. 2000 Sep;38(9):3415-9. doi: 10.1128/JCM.38.9.3415-3419.2000.
10
Comparison of enzyme immunoassay and reverse transcriptase PCR for identification of serotype G9 rotaviruses.酶免疫测定法与逆转录聚合酶链反应用于鉴定G9型轮状病毒的比较
J Clin Microbiol. 1999 Oct;37(10):3187-93. doi: 10.1128/JCM.37.10.3187-3193.1999.
本文引用的文献
1
Infectious gastroenteritis in bone-marrow-transplant recipients.骨髓移植受者的感染性肠胃炎
N Engl J Med. 1982 Apr 29;306(17):1009-2. doi: 10.1056/NEJM198204293061701.
2
Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells.斑块/病灶免疫测定法：一种检测细胞中抗病毒单克隆抗体或其他抗体以及病毒抗原的简单方法。
J Immunol Methods. 1984 Nov 30;74(2):337-44. doi: 10.1016/0022-1759(84)90301-6.
3
Hemagglutination by human rotavirus strains.人轮状病毒株的血凝作用。
J Med Virol. 1984;13(3):215-22. doi: 10.1002/jmv.1890130303.
4
Serological characterisation of human rotaviruses propagated in cell cultures. Brief report.在细胞培养物中增殖的人轮状病毒的血清学特性。简要报告。
Arch Virol. 1984;80(2-3):231-7. doi: 10.1007/BF01310663.
5
Rotaviruses: a review.轮状病毒：综述
Curr Top Microbiol Immunol. 1983;105:123-84. doi: 10.1007/978-3-642-69159-1_3.
6
Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.针对恒河猴轮状病毒两种表面蛋白的单克隆抗体的制备及初步特性分析
J Virol. 1983 Aug;47(2):267-75. doi: 10.1128/JVI.47.2.267-275.1983.
7
Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes.人轮状病毒在细胞培养中的直接分离及其分为四种血清型的特性鉴定。
J Clin Microbiol. 1983 Aug;18(2):310-7. doi: 10.1128/jcm.18.2.310-317.1983.
8
Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus.人轮状病毒W株和DS-1株生长限制、中和作用及亚组特异性的基因编码分配
J Gen Virol. 1983 Feb;64 (Pt 2):313-20. doi: 10.1099/0022-1317-64-2-313.
9
Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation.鉴定编码血凝和蛋白酶增强蚀斑形成的轮状病毒基因。
Virology. 1983 Feb;125(1):194-205. doi: 10.1016/0042-6822(83)90073-9.
10
Serotyping and subgrouping of rotavirus strains by the ELISA test.通过酶联免疫吸附测定试验对轮状病毒毒株进行血清分型和亚组分型。
Arch Virol. 1982;73(3-4):219-30. doi: 10.1007/BF01318076.
Zotero 插件