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用于1型和3型轮状病毒的特异性酶联免疫测定法。

Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.

作者信息

Shaw R D, Stoner-Ma D L, Estes M K, Greenberg H B

出版信息

J Clin Microbiol. 1985 Aug;22(2):286-91. doi: 10.1128/jcm.22.2.286-291.1985.

DOI:10.1128/jcm.22.2.286-291.1985
PMID:2993354
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268377/
Abstract

We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies which react specifically with VP7 of each serotype were identified by hemagglutination inhibition tests, plaque reduction neutralization studies, and solid-phase immunoassays which used wild-type and reassortant strains of rotavirus. An enzyme-linked immunoassay was designed which utilizes two of these antibodies to correctly identify serotype 1 and serotype 3 viruses.

摘要

我们制备了针对两种血清型不同的轮状病毒株的单克隆抗体

Wa,一种源自人类的血清型1病毒,以及恒河猴轮状病毒,一种猿猴血清型3病毒。通过血凝抑制试验、蚀斑减少中和研究以及使用轮状病毒野生型和重配株的固相免疫测定,鉴定出了与每种血清型的VP7特异性反应的单克隆抗体。设计了一种酶联免疫测定法,该方法利用其中两种抗体来正确鉴定血清型1和血清型3病毒。

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Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.用于1型和3型轮状病毒的特异性酶联免疫测定法。
J Clin Microbiol. 1985 Aug;22(2):286-91. doi: 10.1128/jcm.22.2.286-291.1985.
2
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Development of a monoclonal antibody specific for serotype 3 rotavirus strains.针对3型轮状病毒株的单克隆抗体的研制
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本文引用的文献

1
Infectious gastroenteritis in bone-marrow-transplant recipients.骨髓移植受者的感染性肠胃炎
N Engl J Med. 1982 Apr 29;306(17):1009-2. doi: 10.1056/NEJM198204293061701.
2
Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells.斑块/病灶免疫测定法:一种检测细胞中抗病毒单克隆抗体或其他抗体以及病毒抗原的简单方法。
J Immunol Methods. 1984 Nov 30;74(2):337-44. doi: 10.1016/0022-1759(84)90301-6.
3
Hemagglutination by human rotavirus strains.人轮状病毒株的血凝作用。
J Med Virol. 1984;13(3):215-22. doi: 10.1002/jmv.1890130303.
4
Serological characterisation of human rotaviruses propagated in cell cultures. Brief report.在细胞培养物中增殖的人轮状病毒的血清学特性。简要报告。
Arch Virol. 1984;80(2-3):231-7. doi: 10.1007/BF01310663.
5
Rotaviruses: a review.轮状病毒:综述
Curr Top Microbiol Immunol. 1983;105:123-84. doi: 10.1007/978-3-642-69159-1_3.
6
Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.针对恒河猴轮状病毒两种表面蛋白的单克隆抗体的制备及初步特性分析
J Virol. 1983 Aug;47(2):267-75. doi: 10.1128/JVI.47.2.267-275.1983.
7
Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes.人轮状病毒在细胞培养中的直接分离及其分为四种血清型的特性鉴定。
J Clin Microbiol. 1983 Aug;18(2):310-7. doi: 10.1128/jcm.18.2.310-317.1983.
8
Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus.人轮状病毒W株和DS-1株生长限制、中和作用及亚组特异性的基因编码分配
J Gen Virol. 1983 Feb;64 (Pt 2):313-20. doi: 10.1099/0022-1317-64-2-313.
9
Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation.鉴定编码血凝和蛋白酶增强蚀斑形成的轮状病毒基因。
Virology. 1983 Feb;125(1):194-205. doi: 10.1016/0042-6822(83)90073-9.
10
Serotyping and subgrouping of rotavirus strains by the ELISA test.通过酶联免疫吸附测定试验对轮状病毒毒株进行血清分型和亚组分型。
Arch Virol. 1982;73(3-4):219-30. doi: 10.1007/BF01318076.