Biomolecular Chemistry Laboratory, Department of Chemistry, College of Natural Sciences, Chung-Ang University, 84, Huksuk-Ro, Dongjak-Gu, Seoul 06974, Republic of Korea.
Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Food Chem. 2018 Nov 30;267:163-169. doi: 10.1016/j.foodchem.2017.06.084. Epub 2017 Jun 15.
Sweet-tasting proteins may be useful as low-calorie sugar substitutes in foods, beverages, and medicines. Brazzein is an attractive sweetener because of its high sweetness, sugar-like taste, and good stability at high temperature and wide pH ranges. To investigate the bioactivities of brazzein, the antibacterial, antifungal, antioxidant, anti-inflammatory, and anti-allergic activities were determined in vitro. Brazzein showed no antibacterial and antifungal activities, although it showed approximately 45% or greater similarity to defensin, which has antimicrobial effects, and drosomycin, which is used as an antifungal agent. However, brazzein exhibited strong antioxidant effects, showing ABTS radical scavenging activity (IC=12.55μM) and DPPH activity (IC>30μM). Brazzein also showed anti-inflammatory activity and anti-allergic activity in a β-hexosaminidase assay (IC>15μM) and cyclooxygenase-2 inhibition assay (IC=12.62μM), respectively. These results suggest that brazzein has antioxidant, anti-inflammatory, and anti-allergic activities and considerable potential as a functional sweetener.
甜蛋白可用作食品、饮料和药物中的低热量糖替代品。由于具有高甜度、类似糖的味道以及在高温和宽 pH 范围内的良好稳定性，巴西甜蛋白是一种有吸引力的甜味剂。为了研究巴西甜蛋白的生物活性，在体外测定了其抗菌、抗真菌、抗氧化、抗炎和抗过敏活性。巴西甜蛋白没有抗菌和抗真菌活性，尽管它与具有抗菌作用的防御素和用作抗真菌剂的蝇蕈素具有约 45%或更高的相似性。然而，巴西甜蛋白表现出很强的抗氧化作用，表现出 ABTS 自由基清除活性（IC=12.55μM）和 DPPH 活性（IC>30μM）。巴西甜蛋白在β-己糖胺酶测定（IC>15μM）和环氧化酶-2 抑制测定（IC=12.62μM）中也表现出抗炎和抗过敏活性。这些结果表明巴西甜蛋白具有抗氧化、抗炎和抗过敏活性，并且作为功能性甜味剂具有相当大的潜力。
