Vlazny D A, Hyman R W
J Virol. 1985 Oct;56(1):92-101. doi: 10.1128/JVI.56.1.92-101.1985.
Five minority populations of aberrant, varicella-zoster virus (VZV)-derived genomes were identified among the encapsidated DNAs obtained from the nuclear and cytoplasmic fractions of an in vitro infection initiated with a lyophilized sample of the BIKEN VZV vaccine (strain Oka). These were (i) VZV genomes, present within nuclear but not cytoplasmic viral capsids, which had been cleaved at a specific site within the short segment and which were, therefore, 3.15 megadaltons (approximately 4% of the VZV genome length) short of full length; (ii) highly deleted, repetitive VZV genomes which contained the errant cleavage site but not the usual VZV genome terminal sequences; (iii) VZV genomes into which multiples of 1 through 5 defective genome repeat units had been inserted into a homologous site; (iv) VZV genomes with additions of 0.1 or 0.18 megadaltons of DNA at both the terminal and internal ends of the short segment; and (v) VZV DNA which had lost the HindIII restriction site at map position 0.11.
在用冻干的BIKEN水痘-带状疱疹病毒（VZV）疫苗（Oka株）启动的体外感染实验中，从细胞核和细胞质组分获得的衣壳化DNA中鉴定出了五种异常的、源自VZV的基因组群体。这些群体包括：（i）存在于细胞核而非细胞质病毒衣壳中的VZV基因组，其在短片段内的特定位点被切割，因此全长短了3.15兆道尔顿（约占VZV基因组长度的4%）；（ii）高度缺失的、重复的VZV基因组，其包含错误的切割位点但不包含通常的VZV基因组末端序列；（iii）在同源位点插入了1至5个缺陷基因组重复单元倍数的VZV基因组；（iv）在短片段的末端和内部末端均添加了0.1或0.18兆道尔顿DNA的VZV基因组；以及（v）在图谱位置0.11处失去了HindIII限制位点的VZV DNA。
