Takada M, Suzutani T, Yoshida I, Matoba M, Azuma M
Department of Microbiology, Asahikawa Medical College, Japan.
J Clin Microbiol. 1995 Mar;33(3):658-60. doi: 10.1128/jcm.33.3.658-660.1995.
We established a method of identifying varicella-zoster virus (VZV) strains, especially those of the Oka vaccine, in patients with clinical VZV infections. The DNAs of 30 clinically isolated strains and 4 laboratory strains including the Oka vaccine strain and its parent VZV strain, were analyzed by PCR with four sets of primers for the four variable regions, R2, R4, R5, and a region without a PstI site (PS). R4 was unstable in four laboratory VZV strains and was excluded from the study. The other regions were stable in several passages in cell culture. The number of copies in R2 and R5 were distributed from 2 to 13 and from 1 to 3, respectively, in the strains analyzed. The vaccine strain had seven copies in R2 and two copies in R5, and it was PS negative. Among 30 clinical isolates, 3, 23, and 11 strains had the same characteristics as the vaccine strain in R2, R5, and PS, respectively. Therefore, by this method, 97.2% of the isolates were distinguished from the Oka vaccine strain. This strategy will be useful in diagnosing VZV infections induced by the vaccine strain.
我们建立了一种在临床水痘带状疱疹病毒（VZV）感染患者中鉴定VZV毒株，尤其是Oka疫苗毒株的方法。对30株临床分离毒株以及包括Oka疫苗株及其亲本VZV毒株在内的4株实验室毒株的DNA，使用针对四个可变区R2、R4、R5和一个无PstI位点区域（PS）的四组引物进行PCR分析。R4在4株实验室VZV毒株中不稳定，因此被排除在研究之外。其他区域在细胞培养的多次传代中保持稳定。在所分析的毒株中，R2和R5的拷贝数分别分布在2至13个以及1至3个之间。疫苗株在R2中有7个拷贝，在R5中有2个拷贝，且PS呈阴性。在30株临床分离株中，分别有3株、23株和11株在R2、R5和PS方面具有与疫苗株相同的特征。因此，通过这种方法，97.2%的分离株可与Oka疫苗株区分开来。该策略将有助于诊断由疫苗株引起的VZV感染。
