Klintenberg C, Wallgren A, Bjelkenkrantz K, Carstenssen J, Humla S, Nordenskjöld B, Skoog L
Acta Radiol Oncol. 1985 May-Jun;24(3):253-8. doi: 10.3109/02841868509134396.
One hundred and fifty patients with breast carcinoma were examined to compare axillary node status, estrogen receptor level and cellular DNA content as prognostic indicators. Seventy-four per cent of the patients were postmenopausal and forty per cent had axillary node metastases. Estrogen receptor was measured by isoelectric focusing in polyacrylamide gel. DNA was measured in individual cell nuclei by means of Feulgen-acriflavine-sulphate stained imprints. Fifty-two per cent of the tumors had diploid and/or tetraploid DNA pattern, and the rest aneuploid pattern. Axillary node metastases, aneuploid DNA pattern and low level of estrogen receptor were related to recurrence. When introduced into Cox's proportional hazards procedure, axillary nodes and estrogen receptor level but not DNA pattern remained as significant predictors of recurrence.
对150例乳腺癌患者进行检查，以比较腋窝淋巴结状况、雌激素受体水平和细胞DNA含量作为预后指标。74%的患者为绝经后女性，40%有腋窝淋巴结转移。雌激素受体通过聚丙烯酰胺凝胶等电聚焦法测定。DNA通过福尔根-吖啶黄-硫酸盐染色印记在单个细胞核中进行测定。52%的肿瘤具有二倍体和/或四倍体DNA模式，其余为非整倍体模式。腋窝淋巴结转移、非整倍体DNA模式和低水平雌激素受体与复发有关。当纳入考克斯比例风险模型时，腋窝淋巴结和雌激素受体水平而非DNA模式仍是复发的显著预测指标。
