Kobaek-Larsen Morten, Nielsen Dennis Sandris, Kot Witold, Krych Łukasz, Christensen Lars Porskjær, Baatrup Gunnar
Department of Clinical Research, University of Southern Denmark, 5000, Odense, Denmark.
Department of Surgery, Odense University Hospital, 5000, Odense, Denmark.
BMC Res Notes. 2018 Jun 27;11(1):411. doi: 10.1186/s13104-018-3527-y.
(3R)-Falcarinol (FaOH) and (3R,8S)-falcarindiol (FaDOH) have previously been shown to reduce the number of neoplastic lesions and the growth rate of polyps in the colon of azoxymethane (AOM) treated rats. Based on previous investigations, it appears that different mechanisms of actions are involved in the antineoplastic effect of FaOH and FaDOH. One mechanism of action may be related to the antibacterial effect of FaOH and FaDOH and thus their effect on the gut microbiota. This study aimed to determine the effect of FaOH and FaDOH on gut microbiota composition of AOM treated rats.
Azoxymethane treated rats were fed either a standard rat diet or a rat diet supplemented with FaOH and FaDOH. The gut microbiota of AOM-induced rats was determined by 16S rRNA gene-amplicon sequencing. Analysis of fecal cecum samples demonstrated a significant gut microbiota change in rats receiving standard rat diet supplemented with FaOH and FaDOH compared with the control group that only received the rat diet. Comparison of the gut microbiota of rats who developed large neoplasms in the colon with rats without large neoplasms showed that the gut microbiota was significantly different in rats who developed large colon neoplasms compared to rats with no macroscopic colon neoplasms.
先前的研究表明，(3R)-法卡林醇（FaOH）和(3R,8S)-法卡二醇（FaDOH）可减少用氧化偶氮甲烷（AOM）处理的大鼠结肠中的肿瘤性病变数量并降低息肉的生长速率。基于先前的研究，FaOH和FaDOH的抗肿瘤作用似乎涉及不同的作用机制。一种作用机制可能与FaOH和FaDOH的抗菌作用有关，进而与它们对肠道微生物群的影响有关。本研究旨在确定FaOH和FaDOH对AOM处理的大鼠肠道微生物群组成的影响。
给用氧化偶氮甲烷处理的大鼠喂食标准大鼠饲料或补充了FaOH和FaDOH的大鼠饲料。通过16S rRNA基因扩增子测序确定AOM诱导大鼠的肠道微生物群。对粪便盲肠样本的分析表明，与仅接受大鼠饲料的对照组相比，接受补充了FaOH和FaDOH的标准大鼠饲料的大鼠肠道微生物群有显著变化。对结肠中出现大肿瘤的大鼠和未出现大肿瘤的大鼠的肠道微生物群进行比较，结果显示，与无肉眼可见结肠肿瘤的大鼠相比，出现大结肠肿瘤的大鼠肠道微生物群有显著差异。
