i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.
Trends Mol Med. 2018 Aug;24(8):696-708. doi: 10.1016/j.molmed.2018.06.001. Epub 2018 Jun 26.
Intestinal cell models have been widely studied and used to evaluate absorption and metabolism of drugs in the small intestine, constituting valuable tools as a first approach to evaluate the behavior of new drugs. However, such cell models might not be able to fully predict the absorption mechanisms and metabolic pathways of the tested compounds. In recent years, induced pluripotent stem cells (iPSCs) differentiated into enterocyte-like cells have been proposed as more biorelevant intestinal models. In this review, we describe mechanisms underlying the differentiation of iPSCs into enterocyte-like cells, appraise the usefulness of these cells in tridimensional intestinal models, and discuss their suitability to be used in the future for drug screening.
肠细胞模型已被广泛研究和用于评估药物在小肠中的吸收和代谢，作为评估新药行为的初步方法，这些模型构成了有价值的工具。然而，这些细胞模型可能无法完全预测所测试化合物的吸收机制和代谢途径。近年来，诱导多能干细胞（iPSCs）分化为肠上皮样细胞已被提议作为更具生物学相关性的肠道模型。在这篇综述中，我们描述了 iPSCs 分化为肠上皮样细胞的机制，评估了这些细胞在三维肠道模型中的有用性，并讨论了它们在未来用于药物筛选的适用性。
