Department of Biology, Stanford University, Stanford, CA 94305-5020, USA.
Department of Biology, Stanford University, Stanford, CA 94305-5020, USA
Development. 2018 Jul 17;145(14):dev165860. doi: 10.1242/dev.165860.
All multicellular organisms must properly pattern cell types to generate functional tissues and organs. The organized and predictable cell lineages of the leaf enabled us to characterize the role of the MAPK kinase kinase gene in regulating asymmetric cell divisions. We find that YODA genes promote normal stomatal spacing patterns in both and , despite species-specific differences in those patterns. Using lineage tracing and cell fate markers, we show that, unexpectedly, patterning defects in mutants do not arise from faulty physical asymmetry in cell divisions but rather from improper enforcement of alternative cellular fates after division. These cross-species comparisons allow us to refine our understanding of MAPK activities during plant asymmetric cell divisions.
所有多细胞生物都必须正确地形成细胞类型，以产生功能性组织和器官。叶片中组织有序且可预测的细胞谱系使我们能够描述 MAPK 激酶激酶基因在调节不对称细胞分裂中的作用。我们发现，YODA 基因促进了 和 中正常的气孔间距模式，尽管这些模式在物种间存在特异性差异。通过谱系追踪和细胞命运标记，我们发现，出乎意料的是，突变体中的模式缺陷不是由于细胞分裂中物理不对称性的错误，而是由于分裂后替代细胞命运的不当执行。这些跨物种的比较使我们能够完善对植物不对称细胞分裂过程中 MAPK 活性的理解。
