对固始鸡数据的基因组分析揭示了与信阳青壳蛋鸡面部色素沉着相关的基因。

Genomic analysis of GBS data reveals genes associated with facial pigmentation in Xinyang blue-shelled layers.

作者信息

Hou Haobin, Wang Xiaoliang, Zhang Caiyun, Tu Yingying, Lv Wenwei, Cai Xia, Xu Zhigang, Yao Junfeng, Yang Changsuo

机构信息

Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

National Poultry Engineer Research Center, Shanghai 201106, China.

出版信息

Arch Anim Breed. 2020 Dec 18;63(2):483-491. doi: 10.5194/aab-63-483-2020. eCollection 2020.

Facial pigmentation is an important economic trait of chickens, especially for laying hens, which will affect the carcass appearance of eliminated layers. Therefore, identifying the genomic regions and exploring the function of this region that contributes to understanding the variation of skin color traits is significant for breeding. In the study, 291 pure-line Xinyang blue-shelled laying hens were selected, of which 75 were dark-faced chickens and 216 were white-faced chickens. The population was sequenced and typed by GBS genotyping technology. The obtained high-quality SNPs and pigmentation phenotypes were analyzed by a genome-wide association study (GWAS) and a scan. Based on the two analytical methods, we identified a same genomic region (10.70-11.60 Mb) on chromosome 20 with 68 significant SNPs ( ), mapped to 10 known genes, including , , , , , , , , , and , which are associated with dermal hyperpigmentation.

面部色素沉着是鸡的一个重要经济性状，对于蛋鸡来说尤其如此，它会影响淘汰蛋鸡的胴体外观。因此，识别基因组区域并探索该区域对理解肤色性状变异的作用，对于育种具有重要意义。在本研究中，选取了291只纯系信阳青壳蛋鸡，其中75只为黑面鸡，216只为白面鸡。采用GBS基因分型技术对该群体进行测序和分型。利用全基因组关联研究（GWAS）和扫描分析获得的高质量单核苷酸多态性（SNP）和色素沉着表型。基于这两种分析方法，我们在20号染色体上鉴定出一个相同的基因组区域（10.70 - 11.60 Mb），其中有68个显著的SNP（），定位到10个已知基因，包括、、、、、、、、和，这些基因与皮肤色素沉着过度有关。