Gahr M, Blanke R, Speer C P
Biol Neonate. 1985;48(1):15-20. doi: 10.1159/000242147.
Bacterial infections are a major problem in the care for newborn infants. In search for immunological deficits we investigated phagocytosis and killing of staphylococci using polymorphonuclear leukocytes (PMN) isolated from 2 ml of venous blood. Phagocytosis of PMN from preterm (n = 10) and newborn infants (n = 9; mean birth weights 1,949 and 3,523 g, respectively) was not different from that of adult PMN (n = 14). Killing capacity of PMN from preterm infants was markedly impaired compared to term newborn infants and to adult PMN. We found similar rates of superoxide anion production and similar times for activation in response to phorbolmyristate acetate stimulation. Our study gives further evidence that PMN from term newborn infants have normal phagocytotic and bactericidal capacity. In PMN from preterm newborn infants, however, the bactericidal capacity is diminished similar to newborn infants under stress as described earlier by others.
细菌感染是新生儿护理中的一个主要问题。为了寻找免疫缺陷，我们使用从2毫升静脉血中分离出的多形核白细胞（PMN）研究了对葡萄球菌的吞噬作用和杀伤能力。早产（n = 10）和新生儿（n = 9；平均出生体重分别为1949克和3523克）的PMN的吞噬作用与成人PMN（n = 14）的吞噬作用没有差异。与足月新生儿和成人PMN相比，早产婴儿PMN的杀伤能力明显受损。我们发现超氧阴离子产生率相似，对佛波酯乙酸盐刺激的激活时间也相似。我们的研究进一步证明，足月新生儿的PMN具有正常的吞噬和杀菌能力。然而，早产新生儿的PMN的杀菌能力减弱，类似于其他研究人员之前描述的处于应激状态的新生儿。
