1Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
2Institute of Virology, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
J Gen Virol. 2019 Feb;100(2):301-307. doi: 10.1099/jgv.0.001197. Epub 2018 Dec 12.
Ascoviruses are enveloped, circular, double-stranded DNA viruses that can effectively control the appetite of lepidopteran larvae, thereby reducing the consequent damage and economic losses to crops. In this study, the virion of a sequenced Heliothis virescens ascovirus 3i (HvAV-3i) strain was used to perform proteomic analysis using both in-gel and in-solution digestion. A total of 81 viral proteins, of which 67 were associated with the virions, were identified in the proteome of HvAV-3i virions. Among these proteins, 23 with annotated functions were associated with DNA/RNA metabolism/transcription, virion assembly, sugar and lipid metabolism, signalling, cellular homoeostasis and cell lysis. Twenty-one viral membrane proteins were also identified. Some of the minor 'virion' proteins identified may be non-virion contaminants of viral proteins synthesized during replication, identified by more recent and highly sensitive methods. The extensive identification of the ascoviral proteome will establish a foundation for further investigation of ascoviral replication and infection.
杆状病毒是有包膜的、环状的、双链 DNA 病毒，能有效控制鳞翅目幼虫的食欲，从而减少对农作物的后续损害和经济损失。在这项研究中，使用经测序的烟实夜蛾杆状病毒 3i（HvAV-3i）株的病毒粒子，通过胶内和溶液内消化进行蛋白质组分析。在 HvAV-3i 病毒粒子的蛋白质组中鉴定出 81 种病毒蛋白，其中 67 种与病毒粒子有关。在这些蛋白中，有 23 种具有注释功能的蛋白与 DNA/RNA 代谢/转录、病毒粒子组装、糖和脂质代谢、信号转导、细胞内稳态和细胞裂解有关。还鉴定出 21 种病毒膜蛋白。一些鉴定出的较小的“病毒粒子”蛋白可能是复制过程中合成的病毒蛋白的非病毒粒子污染物，这些蛋白是通过最近更灵敏的方法鉴定出来的。对杆状病毒蛋白质组的广泛鉴定将为进一步研究杆状病毒的复制和感染奠定基础。
