Chen Gong, Liu Hang, Mo Bo-Cheng, Hu Jue, Liu Shuang-Qing, Bustos-Segura Carlos, Xue Jing, Wang Xing
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China.
College of Plant Protection, Hunan Agricultural University, Changsha, China.
Front Physiol. 2020 Mar 5;11:93. doi: 10.3389/fphys.2020.00093. eCollection 2020.
Although the cotton bollworm has traditionally been controlled by application of chemical pesticides, chemical control selects for resistance, pollutes the environment, and endangers human health. New methods for controlling are therefore needed. 3i (HvAV-3i) is a recently identified virus of the lepidopteran larvae. We tested the effects of HvAV-3i on larvae following oral ingestion of HvAV-3i-containing hemolymph (about 1.0 × 10 virus genome copies per larvae) and following injection of HvAV-3i-containing hemolymph by insertion of a needle. Following oral ingestion, first-instar to fifth-instar larvae grew and developed normally. Following needle injection, in contrast, the corrected mortality of third and fourth instars was 88.9 ± 2.1 and 93.7 ± 3.4%, respectively. Food intake was significantly lower for larvae injected with virus-containing hemolymph than with virus-free hemolymph. Larvae injected with virus-containing hemolymph had extended survival times and could not complete the pre-pupal stage. These results indicate that inoculation of HvAV-3i via needle injection, but not via oral ingestion, significantly reduced the growth and development of larvae.
虽然传统上棉铃虫是通过施用化学农药来控制的，但化学防治会导致害虫产生抗药性，污染环境，并危害人类健康。因此，需要新的防治方法。HvAV-3i是最近发现的一种鳞翅目幼虫病毒。我们测试了口服含HvAV-3i的血淋巴（每只幼虫约1.0×10个病毒基因组拷贝）以及通过插入针头注射含HvAV-3i的血淋巴后，HvAV-3i对棉铃虫幼虫的影响。口服后，一龄至五龄幼虫生长发育正常。相比之下，注射后，三龄和四龄幼虫的校正死亡率分别为88.9±2.1%和93.7±3.4%。注射含病毒血淋巴的幼虫的食物摄入量明显低于注射无病毒血淋巴的幼虫。注射含病毒血淋巴的幼虫存活时间延长，无法完成蛹前期。这些结果表明，通过针头注射而非口服接种HvAV-3i可显著降低棉铃虫幼虫的生长发育。
