Wang Xiaodan, Yan Yongping, Yang Liu, Li Mu, Zhong Xiuhui
J Tradit Chin Med. 2016 Dec;36(6):737-42. doi: 10.1016/s0254-6272(17)30008-0.
To explore the effect of quercetin on the expressions of Bcl-2/Bax apoptotic proteins in endometrial cells in mice with abortion induced by lipopolysaccharide.
For in vivo experiment, twenty five Kunming mice were randomly divided into five groups at day 4 of pregnancy, with 5 mice per group. The mice were treated with lipopolysaccharide (LPS) through tail vein intravenous injection at day 4 of pregnancy, followed by different concentrations of quercetin by oral gavage consecutively at days 5 to 6 of pregnancy. On day 7 of gestation, the mice were sacrificed and the histopathological changes of the uterus tissues were observed. Immunohistochemical staining was applied to the detection of Bcl-2/Bax apoptotic proteins in the endometrial cells. For in vitro experiment, the primary endometrial cells were cultured using a uterus tissue mass culturing method sampled at day 4.5 of pregnancy. The cells were treated with LPS with or without different dosages of quercetin, respectively, for 12 h after 80% confluence. The expression of Bcl-2/Bax apoptotic proteins were detected by western blotting.
Both the in vivo and in vitro experiments showed decreased expression of Bcl-2 and enhanced expression of Bax after LPS treatment, leading to a decreased Bcl-2/Bax ratio. The expression of Bcl-2 significantly increased while the expression of Bax was significantly elevated, in the LPS plus quercetin group compared to the LPS only group.
These results suggest that quercetin has protective effect by partially regulating the expression of Bcl-2/Bax proteins, which in turn inhibits endometrial cell apoptosis and benefits the embryo implantation.
探讨槲皮素对脂多糖诱导流产小鼠子宫内膜细胞中Bcl-2/Bax凋亡蛋白表达的影响。
体内实验，25只昆明小鼠于妊娠第4天随机分为5组，每组5只。在妊娠第4天经尾静脉注射脂多糖（LPS）处理小鼠，随后在妊娠第5至6天连续经口灌胃不同浓度的槲皮素。在妊娠第7天处死小鼠，观察子宫组织的组织病理学变化。采用免疫组织化学染色检测子宫内膜细胞中Bcl-2/Bax凋亡蛋白。体外实验，采用妊娠第4.5天采集的子宫组织块培养法培养原代子宫内膜细胞。细胞在80%汇合后分别用LPS加或不加不同剂量的槲皮素处理12小时。通过蛋白质印迹法检测Bcl-2/Bax凋亡蛋白的表达。
体内和体外实验均显示，LPS处理后Bcl-2表达降低，Bax表达增强，导致Bcl-2/Bax比值降低。与仅LPS组相比，LPS加槲皮素组Bcl-2表达显著增加，Bax表达显著升高。
这些结果表明，槲皮素通过部分调节Bcl-2/Bax蛋白的表达发挥保护作用，进而抑制子宫内膜细胞凋亡并有利于胚胎着床。
