Dai Min, Peng Cheng, Sun Fenghui
J Tradit Chin Med. 2016 Dec;36(6):799-804. doi: 10.1016/s0254-6272(17)30018-3.
To evaluate the anti-infectious efficacy of essential oil extracted from Caoguo (Fructus Tsaoko).
Minimum inhibitory concentrations (MICs) against clinical isolates of three extracts and the essential oil from Caoguo (Fructus Tsaoko) were determined by the agar dilution method. The antiinfectious efficacy of the essential oil was evaluated using a mouse peritonitis model which was infected with Staphylococcus aureus or Escherichia coli. The chemical components of the essential oil were identified.
The results showed that the essential oil exhibited strong antibacterial activity in vitro, with MICs ranging from 22.49 to 1438.91 μg/mL. The results of in vivo anti-infectious efficacy showed that the Caoguo (Fructus Tsaoko) essential oil can protect the mice from Staphylococcus aureus or Escherichia coli infection. The compositions of the essential oil and relative component percentages were examined. A total of 32 compounds, were identified. The major compounds of essential oil were 1, 8-cineole (25.92%) and geraniol (13.69%).
Our findings suggest that Caogu (Fructus Tsaoko) essential oil has broad-spectrum antibacterial properties. It warrants further investigation as an antibacterial agent targeting some bacterium with multi-drug resistance.
评价草果挥发油的抗感染疗效。
采用琼脂稀释法测定草果三种提取物及挥发油对临床分离菌的最低抑菌浓度（MIC）。采用小鼠腹膜炎模型，分别感染金黄色葡萄球菌或大肠杆菌，评价挥发油的抗感染疗效。鉴定挥发油的化学成分。
结果表明，挥发油在体外表现出较强的抗菌活性，MIC范围为22.49至1438.91μg/mL。体内抗感染疗效结果表明，草果挥发油可保护小鼠免受金黄色葡萄球菌或大肠杆菌感染。检测了挥发油的组成及相对成分百分比。共鉴定出32种化合物。挥发油的主要成分是1,8-桉叶素（25.92%）和香叶醇（13.69%）。
我们的研究结果表明，草果挥发油具有广谱抗菌特性。作为一种针对某些多重耐药细菌的抗菌剂，值得进一步研究。
