通过氯化锂沉淀法从经葡聚糖硫酸钠（DSS）处理的小鼠组织中纯化总RNA。
Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation.
作者信息
Viennois Emilie, Tahsin Anika, Merlin Didier
机构信息
Institute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA, USA.
Veterans Affairs Medical Center, Decatur, GA, USA.
出版信息
Bio Protoc. 2018 May 5;8(9). doi: 10.21769/BioProtoc.2829.
Abstract
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.
摘要
我们已经开发出一种从经硫酸葡聚糖钠（DSS）处理的小鼠组织中纯化RNA的方案。该方法依靠用氯化锂连续沉淀，可防止在经DSS处理的组织中观察到的对定量逆转录聚合酶链反应（q-RT-PCR）的下游抑制。
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本文引用的文献
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