Department of Infectious Disease, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
Inflammation. 2018 Oct;41(5):1755-1761. doi: 10.1007/s10753-018-0818-3.
In the present study, we used the human umbilical vein endothelial cells (HUVECs) to investigate the anti-inflammatory effects and mechanism of taraxasterol on vascular inflammation. HUVECs were pre-treated with taraxasterol 1 h before lipopolysaccharide (LPS) treatment. The concentrations of TNF-α, IL-8, PGE2, and NO were measured. The expression of VCAM-1, ICAM-1, iNOS, COX-2, NF-κB, and LXRα was detected by western blot analysis. The results showed that taraxasterol not only reduced the production of TNF-α, IL-8, PGE2, and NO induced by LPS, but also reduced the expression of iNOS and COX-2. Taraxasterol also suppressed LPS-induced NF-κB activation and VCAM-1 and ICAM-1 expression. Furthermore, taraxasterol concentration-dependently increased the expression of LXRα. The inhibition of taraxasterol on TNF-α, IL-8, PGE2, and NO production can be reversed by geranylgeranyl diphosphate (GGPP, the LXRα inhibitor). Here, we found that taraxasterol inhibited vascular inflammation through activating LXRα.
在本研究中，我们使用人脐静脉内皮细胞（HUVEC）来研究蒲公英甾醇对血管炎症的抗炎作用和机制。HUVEC 在脂多糖（LPS）处理前用蒲公英甾醇预处理 1 小时。测量 TNF-α、IL-8、PGE2 和 NO 的浓度。通过 Western blot 分析检测 VCAM-1、ICAM-1、iNOS、COX-2、NF-κB 和 LXRα 的表达。结果表明，蒲公英甾醇不仅降低了 LPS 诱导的 TNF-α、IL-8、PGE2 和 NO 的产生，还降低了 iNOS 和 COX-2 的表达。蒲公英甾醇还抑制 LPS 诱导的 NF-κB 激活和 VCAM-1 和 ICAM-1 的表达。此外，蒲公英甾醇浓度依赖性地增加了 LXRα 的表达。LXRα 抑制剂香叶基香叶基二磷酸（GGPP）可逆转蒲公英甾醇对 TNF-α、IL-8、PGE2 和 NO 产生的抑制作用。在这里，我们发现蒲公英甾醇通过激活 LXRα抑制血管炎症。
