Perussia B, Fanning V, Trinchieri G
Nat Immun Cell Growth Regul. 1985;4(3):120-37.
In this report, we characterize the major human peripheral blood nonadherent mononuclear cell subset that is responsible for the production in vitro of alpha-interferon (alpha IFN) in response to influenza, Sendai, and Newcastle disease viruses. Using a panel of anti-monocyte, anti-B, anti-T and anti-natural killer cell monoclonal antibodies to purify and recover both positive and negative cell populations, we show that the major alpha IFN-producing cells are HLA-DR(+) cells with no other surface markers characteristic of either lymphocytes or myelomonocytic cells. These cells copurify, on Percoll density gradients, with cells mediating NK activity, but the cell population responsible for alpha IFN production can be distinguished unambiguously from NK cells based on the former's reactivity with an anti-HLA-DR monoclonal antibody, the lack of reactivity with antibodies that detect the low-affinity receptor for IgG on human natural killer cells and granulocytes, and the inability to mediate spontaneous cytotoxicity. Double immunofluorescence assays indicate that cells of this subset, the lineage of which is as yet undetermined but which might be related to dendritic cells, constitute a minor proportion (approximately 1-1.5%) of the nonadherent mononuclear cells from healthy donors.
在本报告中，我们鉴定了主要的人类外周血非黏附单核细胞亚群，该亚群负责在体外对流感病毒、仙台病毒和新城疫病毒产生α干扰素（α IFN）。我们使用一组抗单核细胞、抗B细胞、抗T细胞和抗自然杀伤细胞单克隆抗体来纯化和回收阳性及阴性细胞群体，结果表明，主要产生α IFN的细胞是HLA - DR(+)细胞，没有淋巴细胞或髓单核细胞特有的其他表面标志物。这些细胞在Percoll密度梯度上与介导NK活性的细胞共同纯化，但基于前者与抗HLA - DR单克隆抗体的反应性、与检测人类自然杀伤细胞和粒细胞上IgG低亲和力受体的抗体缺乏反应性以及无法介导自发细胞毒性，可以明确区分负责α IFN产生的细胞群体与NK细胞。双重免疫荧光分析表明，该亚群的细胞在健康供体的非黏附单核细胞中占比很小（约1 - 1.5%），其谱系尚未确定，但可能与树突状细胞有关。
