Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany.
Syst Appl Microbiol. 2018 Nov;41(6):564-569. doi: 10.1016/j.syapm.2018.06.002. Epub 2018 Jun 8.
Two strains (pedersoli and girotti) of a new species of bacteria were isolated from the preen glands of wild Egyptian geese (Alopochen aegyptiacus) from the river Neckar in southern Germany in two subsequent years. The strains were lipophilic, fastidious, Gram-positive rods and belonged to the genus Corynebacterium. Phylogenetically, the isolates were most closely related to Corynebacterium falsenii DSM 44353 which has been found to be associated with birds before. 16S rRNA gene sequence similarity to all known Corynebacterium spp. was significantly <97%. Corresponding values of rpoB showed low levels of similarity <87% and ANIb was <73%. G+C content of the genomic DNA was 65.0mol% for the type strain of the goose isolates, as opposed to 63.2mol% in Corynebacterium falsenii. MALDI-TOF MS analysis of the whole-cell proteins revealed patterns clearly different from the related species, as did biochemical tests, and polar lipid profiles. We therefore conclude that the avian isolates constitute strains of a new species, for which the name Corynebacterium heidelbergense sp. nov. is proposed. The type strain is pedersoli (=DSM 104638=LMG 30044).
两株（pedersoli 和 girotti）新分离的细菌菌株来自德国南部内卡河野生埃及鹅（Alopochen aegyptiacus）的尾脂腺，这两株菌均为亲脂、挑剔的革兰氏阳性杆菌，属于棒状杆菌属。从系统发育上看，这些分离株与以前发现与鸟类有关的假性棒状杆菌（Corynebacterium falsenii DSM 44353）最为密切相关。16S rRNA 基因序列与所有已知的 Corynebacterium spp. 的相似性显著<97%。rpoB 对应的数值显示出低水平的相似性<87%，ANIb 则<73%。基因组 DNA 的 GC 含量为鹅分离株的模式株的 65.0mol%，而假性棒状杆菌的为 63.2mol%。全细胞蛋白的 MALDI-TOF MS 分析结果显示，与相关种的模式明显不同，生化试验和极性脂谱也是如此。因此，我们得出结论，这些禽源分离株构成了一个新种的菌株，我们提议将其命名为海德堡棒状杆菌（Corynebacterium heidelbergense sp. nov.）。模式株为 pedersoli（=DSM 104638=LMG 30044）。
