Muggeridge M I, Fraser N W
J Virol. 1986 Sep;59(3):764-7. doi: 10.1128/JVI.59.3.764-767.1986.
After corneal inoculation, herpes simplex virus type 1 replicates in the mouse eye, trigeminal ganglia, and brainstem, producing first an acute and then a latent infection. Previous work from this laboratory focused on the structure of the viral DNA in this system. We have now examined the structure of the viral genome at the chromosome level by using micrococcal nuclease digestion. Studies with disaggregated cell preparations made from the brainstems of acutely infected mice show that the majority of the viral DNA is in a nonnucleosomal form; however, a nucleosomelike fraction was also consistently detected. A similar result was obtained for viral DNA in herpes simplex virus type 1-infected C1300 (clone NA) neuroblastoma cells (a neuronal cell line).
角膜接种后，1型单纯疱疹病毒在小鼠眼睛、三叉神经节和脑干中复制，首先产生急性感染，然后是潜伏感染。该实验室之前的工作集中在这个系统中病毒DNA的结构。现在我们通过使用微球菌核酸酶消化在染色体水平上研究了病毒基因组的结构。对急性感染小鼠脑干制备的分散细胞制剂的研究表明，大多数病毒DNA呈非核小体形式；然而，也一直检测到类似核小体的部分。在1型单纯疱疹病毒感染的C1300（克隆NA）神经母细胞瘤细胞（一种神经元细胞系）中的病毒DNA也得到了类似的结果。
