Pandey Sunil, Goel Mahima, Nagpal Ravi, Kar Ankita, Rapsang Eliezer, Matani Priya
Department of Pedodontics and Preventive Dentistry Government Dental College, Raipur, Chhattisgarh, India, Phone: +919424259047 e-mail:
Department of Oral and Maxillofacial Surgery, Maharaja Ganga Singh Dental College & Research Centre, Sri Ganganagar Rajasthan, India.
J Contemp Dent Pract. 2018 Jun 1;19(6):651-655.
The occurrence of dental caries has become quite a common phenomenon nowadays. The varying levels of salivary secretory immunoglobulin A (SIgA) usually determine the progression of caries. The present study was aimed to determine the correlation between SIgA and mutans-specific antigen SIgA in children having different caries status. Scanning electron microscopic analysis was also completed to correlate the results.
This study comprised 60 subjects, who were divided into three groups depending on caries status. In all, saliva was collected to determine the level of SIgA and mutans-specific antigen SIgA using enzyme linked immunosorbent assay (ELISA). The World Health Organization (WHO) criteria and method were used to evaluate dental caries. Bradford reagent was used to evaluate the levels of protein in the antigen. Furthermore, 20 sections of enamel were randomly obtained to estimate the severity of caries development among groups.
Categorical characteristics among all groups were compared by basic statistical analysis and Chi-squared test. Mean age (years) was found to be 9.214 ± 2.28, 9.5 ± 2.51, and 10.2 ± 2.35 in groups I, II, and III respectively. Mutans-specific IgA level (|jg/mL) was 34.63 ± 7.46, 28.24 ± 4.52, and 23.56 ± 1.62 in groups I, II, and III respectively. Total SIgA (jg/mL) was 142.53 ± 22.4, 186.10 ± 24.70, and 214.8 ± 27.56 in groups I, II, and III respectively. Caries index was 6.74 ± 2.16, 2.32 ± 0.86, and 0 ± 0 in groups I, II, and III respectively.
Immunoglobulin A is dominantly present in saliva and it plays a significant role in prevention of dental caries. Hence, dental caries is more likely to develop in subjects with low level of salivary IgA (high caries index).
A low level of IgA may be associated with a high risk of developing dental caries. This association may possibly be useful in predicting the future caries status. Accordingly, suitable caries-preventive measures can be selected and employed.
如今，龋齿的发生已成为一种相当常见的现象。唾液分泌型免疫球蛋白A（SIgA）水平的变化通常决定龋齿的发展进程。本研究旨在确定不同龋齿状况儿童的SIgA与变形链球菌特异性抗原SIgA之间的相关性。还完成了扫描电子显微镜分析以关联结果。
本研究包括60名受试者，根据龋齿状况分为三组。总共收集唾液，使用酶联免疫吸附测定（ELISA）来测定SIgA和变形链球菌特异性抗原SIgA的水平。采用世界卫生组织（WHO）的标准和方法来评估龋齿。使用Bradford试剂评估抗原中的蛋白质水平。此外，随机获取20个牙釉质切片以估计各组中龋齿发展的严重程度。
通过基本统计分析和卡方检验比较了所有组之间的分类特征。发现I组、II组和III组的平均年龄（岁）分别为9.214±2.28、9.5±2.51和10.2±2.35。I组、II组和III组的变形链球菌特异性IgA水平（|jg/mL）分别为34.63±7.46、28.24±4.52和23.56±1.62。I组、II组和III组的总SIgA（jg/mL）分别为142.53±22.4、186.10±24.70和214.8±27.56。I组、II组和III组的龋齿指数分别为6.74±2.16、2.32±0.86和0±0。
免疫球蛋白A主要存在于唾液中，在预防龋齿方面发挥着重要作用。因此，唾液IgA水平低（龋齿指数高）的受试者更易发生龋齿。
低水平的IgA可能与患龋齿的高风险相关。这种关联可能有助于预测未来的龋齿状况。据此，可以选择并采用合适的龋齿预防措施。
