Wang Hongwei, Li Hailong, Chen Fengqin, Luo Jun, Gu Jing, Wang Huping, Wu Hongyan, Xu Yan
J Tradit Chin Med. 2017 Apr;37(2):229-5. doi: 10.1016/s0254-6272(17)30049-3.
To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.
Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells. Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.
In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner. Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma (Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the mRNA and protein level.
Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.
评估黄芩苷对人胃癌细胞的作用，包括诱导凋亡的作用，并探讨其潜在的作用机制。
进行细胞增殖和凋亡检测，以研究黄芩苷对人胃癌BGC - 823和MGC - 803细胞的抗增殖作用。采用实时定量聚合酶链反应和蛋白质印迹分析来阐明黄芩苷抗肿瘤特性的分子机制。
在分别用80、120和160 μmol/L黄芩苷处理48小时的BGC - 823和MGC - 803胃癌细胞中，噻唑蓝比色法显示黄芩苷以剂量依赖性方式显著抑制细胞增殖，而流式细胞术分析表明黄芩苷也以剂量依赖性方式诱导凋亡。此外，黄芩苷在mRNA和蛋白质水平上均上调了半胱天冬酶 - 3、半胱天冬酶 - 9和B细胞淋巴瘤相关X蛋白的表达，并下调了Bcl - 2的表达。
黄芩苷通过诱导癌细胞凋亡具有作为胃癌治疗药物的潜力。
