Luo Lu-Yun, Jin De-Cai, Zuo Hui, Zhang Zhuo, Tan Xin-Qiu, Zhang De-Yong, Lu Xiang-Yang, Liu Yong
College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Institute of Hunan Plant Protection, Hunan Academy of Agricultural Science, Changsha 410125, China.
Huan Jing Ke Xue. 2017 Feb 8;38(2):735-742. doi: 10.13227/j.hjkx.201606059.
The use of biological pesticide can greatly reduce the soil pollution in the environment. Exploring the effect of biological pesticide on community diversity and distribution of pathogenic bacteria will provide theoretic basis for subsequent researches on biological pesticide micro-ecological control. In order to explore the microbial ecological mechanism of pepper phytophthora blight, this research compared the difference of microbial diversity between rhizosphere soil of infected and healthy plants, and the effects of PSB06 on microbial diversities of plant rhizosphere soil were investigated using Illumina MiSeq sequencing technology. The results showed that there was less difference in the microbial diversity from the same soil between the seventh day and the fourteenth day. The microbial diversity of rhizosphere soil of healthy plants was higher than that of rhizosphere soil of infected plants. The soil sprayed with PSB06 exhibited the highest diversity. Moreover, the abundance of Actinomycetes in the rhizosphere soil of healthy plants was higher than that of infected plants, and the highest abundance of Actinomycetes was observed in the soil sprayed with PSB06. The microbial diversity between rhizosphere soil of infected and healthy plants was significantly different. Spraying PSB06 could significantly alter the microbial community structure of the soil. It could also increase the diversity of microorganism and the abundance of Actinomycetes in the soil.
生物农药的使用能够极大地减少环境中的土壤污染。探究生物农药对病原菌群落多样性及分布的影响，将为后续生物农药微生态防治研究提供理论依据。为探究辣椒疫霉病的微生物生态机制，本研究比较了感病植株与健康植株根际土壤微生物多样性的差异，并采用Illumina MiSeq测序技术研究了PSB06对植物根际土壤微生物多样性的影响。结果表明，同一土壤在第7天和第14天的微生物多样性差异较小。健康植株根际土壤的微生物多样性高于感病植株根际土壤。喷施PSB06的土壤表现出最高的多样性。此外，健康植株根际土壤中放线菌的丰度高于感病植株，喷施PSB06的土壤中放线菌丰度最高。感病植株与健康植株根际土壤的微生物多样性存在显著差异。喷施PSB06可显著改变土壤微生物群落结构，还可增加土壤中微生物的多样性及放线菌的丰度。
