College of Horticulture, Northwest A&F University, Shaanxi 712100, PR China.
College of Horticulture, Northwest A&F University, Shaanxi 712100, PR China.
Genomics. 2019 Sep;111(5):1043-1052. doi: 10.1016/j.ygeno.2018.06.007. Epub 2018 Jul 13.
Zygaenidae comprises >1036 species, including many folivorous pests in agriculture. In the present study, the complete mitochondrial genome (mitogenome) of a major pest of tea trees, Eterusia aedea was determined. The 15,196-bp circular genome contained the common set of 37 mitochondrial genes (including 13 protein-coding genes, two rRNA genes, and 22 tRNA genes) and exhibited the similar genomic features to reported Zygaenidae mitogenome. Comparative analyses of Zygaenidae mitogenomes showed a typical evolutionary trend of lepidopteran mitogenomes. In addition, we also investigated the gene order of lepidopteran mitogenomes and proposed that the novel gene order trnA-trnR-trnN-trnE-trnS-trnF from Zygaenidae and Gelechiidae and most other gene rearrangements of this tRNA cluster evolved independently. Finally, the mitogenomic phylogeny of Lepidoptera was reconstructed based on multiple mitochondrial datasets. And all the phylogenetic results revealed the sister relationships of Cossoidea and Zygaenoidea with both BI and ML methods, which is the first stable mitogenomic evidence for this clade.
眼蝶科包含超过 1036 种物种，包括农业中的许多食叶害虫。本研究测定了茶树主要害虫茶褐带卷蛾的完整线粒体基因组（mitogenome）。这个 15196 碱基对的圆形基因组包含了常见的 37 个线粒体基因（包括 13 个蛋白编码基因、两个 rRNA 基因和 22 个 tRNA 基因），并表现出与已报道的眼蝶科线粒体基因组相似的基因组特征。对眼蝶科线粒体基因组的比较分析显示了鳞翅目线粒体基因组的典型进化趋势。此外，我们还研究了鳞翅目线粒体基因组的基因排列，并提出了来自眼蝶科和卷蛾科的新型 trnA-trnR-trnN-trnE-trnS-trnF 基因排列以及该 tRNA 簇的大多数其他基因重排是独立进化的。最后，基于多个线粒体数据集重建了鳞翅目线粒体基因组的系统发育关系。所有的系统发育结果都显示出了 Cossoidea 和 Zygaenoidea 的姐妹关系，无论是在 BI 还是 ML 方法中，这是该分支的第一个稳定的线粒体基因组证据。
