NanoLund and Solid State Physics , Lund University , Box 118, Lund SE-221 00 , Sweden.
Department of Chemistry and Biomedical Sciences , Linnaeus University , Kalmar SE-391 82 , Sweden.
Langmuir. 2018 Jul 31;34(30):8777-8784. doi: 10.1021/acs.langmuir.8b01415. Epub 2018 Jul 18.
Molecular motor-based nanodevices require organized cytoskeletal filament guiding along motility-promoting tracks, confined by motility-inhibiting walls. One way to enhance motility quality on the tracks, particularly in terms of filament velocity but also the fraction of motile filaments, is to optimize the surface hydrophobicity. We have investigated the potential to achieve this for the actin-myosin II motor system on trimethylchlorosilane (TMCS)-derivatized SiO surfaces to be used as channel floors in nanodevices. We have also investigated the ability to supress motility on two new polymer resists, TU7 (for nanoimprint lithography) and CSAR 62 (for electron beam and deep UV lithography), to be used as channel walls. We developed a chemical-vapor deposition tool for silanizing SiO surfaces in a controlled environment to achieve different surface hydrophobicities (measured by water contact angle). In contrast to previous work, we were able to fabricate a wide range of contact angles by varying the silanization time and chamber pressure using only one type of silane. This resulted in a significant improvement of the silanization procedure, producing a predictable contact angle on the surface and thereby predictable quality of the heavy meromyosin (HMM)-driven actin motility with regard to velocity. We observed a high degree of correlation between the filament sliding velocity and contact angle in the range 10-86°, expanding the previously studied range. We found that the sliding velocity on TU7 surfaces was superior to that on CSAR 62 surfaces despite similar contact angles. In addition, we were able to suppress the motility on both TU7 and CSAR 62 by plasma oxygen treatment before silanization. These results are discussed in relation to previously proposed surface adsorption mechanisms of HMM and their relationship to the water contact angle. Additionally, the results are considered for the development of actin-myosin based nanodevices with superior performance with respect to actin-myosin functionality.
基于分子马达的纳米器件需要有组织的细胞骨架丝沿着促进运动的轨道引导，同时受到运动抑制壁的限制。提高轨道上运动质量的一种方法，特别是在丝速度方面，但也包括运动丝的分数方面，是优化表面疏水性。我们已经研究了在三甲基氯硅烷（TMCS）衍生的 SiO 表面上实现这一点的潜力，这些表面将用作纳米器件中的通道地板。我们还研究了在两种新的聚合物抗蚀剂 TU7（用于纳米压印光刻）和 CSAR 62（用于电子束和深紫外光刻）上抑制运动的能力，这些抗蚀剂将用作通道壁。我们开发了一种化学气相沉积工具，用于在受控环境中硅烷化 SiO 表面，以实现不同的表面疏水性（通过水接触角测量）。与以前的工作不同，我们仅使用一种类型的硅烷，通过改变硅烷化时间和腔室压力，能够制造出广泛的接触角。这导致硅烷化过程得到了显著改进，在表面上产生了可预测的接触角，从而可预测重酶解肌球蛋白（HMM）驱动的肌动蛋白运动的质量，包括速度。我们观察到在 10-86°范围内，滑动速度与接触角之间具有高度相关性，扩展了以前研究的范围。我们发现，尽管接触角相似，但 TU7 表面上的滑动速度优于 CSAR 62 表面上的滑动速度。此外，我们能够通过在硅烷化之前进行等离子体氧气处理来抑制 TU7 和 CSAR 62 上的运动。这些结果与以前提出的 HMM 表面吸附机制及其与水接触角的关系进行了讨论。此外，还考虑了这些结果在开发具有卓越肌动蛋白-肌球蛋白功能的基于肌动蛋白-肌球蛋白的纳米器件方面的应用。
