Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná 86057-970, Brazil.
Academic Department of Food, Universidade Tecnológica Federal do Paraná, Avenida dos Pioneiros, 3131, Londrina, Paraná 86036-370, Brazil.
Toxins (Basel). 2018 Jul 4;10(7):276. doi: 10.3390/toxins10070276.
Deoxynivalenol (DON) is a mycotoxin that affects the intestinal morphology of animals, impairing nutrient intake and growth. On the other hand, dietary supplementation with functional oligosaccharides as chito-oligosaccharides (COS) has shown positive effects on the intestinal health of piglets. Therefore, the objective of the present study was to evaluate the effect of low doses of COS in preventing DON-induced intestinal histological changes, using a swine jejunal explant technique. The intestinal explants were incubated at 37 °C in culture medium for 4 h and exposed to the following treatments: (a) control (only culture medium), (b) DON (10 µM), (c) 25COS (0.025 mg·mL of COS); (d) 50COS (0.05 mg·mL of COS); (e) 25COS plus DON (25COS + DON); (f) 50COS plus DON (50COS + DON). Explants exposed to COS presented intestinal morphology similar to control samples. DON induced a significant decrease in the histological score as a consequence of moderate to severe histological changes (apical necrosis, villi atrophy, and fusion) and a significant decrease in morphometric parameters (villi height, crypt depth, villi height:crypt depth ratio, and goblet cells density). The intestinal morphology of samples exposed to COS + DON remained similar to DON treatment. In conclusion, low levels of COS did not counteract DON-induced intestinal lesions.
脱氧雪腐镰刀菌烯醇（DON）是一种真菌毒素，会影响动物的肠道形态，从而损害营养物质的摄入和生长。另一方面，功能性寡糖（如壳寡糖（COS））作为膳食补充剂已被证明对仔猪的肠道健康有积极影响。因此，本研究旨在使用猪空肠离体技术来评估低剂量 COS 预防 DON 诱导的肠道组织学变化的效果。将肠段在 37℃的培养箱中用培养基孵育 4 小时，并暴露于以下处理中：（a）对照（仅培养基），（b）DON（10 μM），（c）25COS（0.025 mg·mL 的 COS）；（d）50COS（0.05 mg·mL 的 COS）；（e）25COS+DON（25COS+DON）；（f）50COS+DON（50COS+DON）。暴露于 COS 的肠段呈现出与对照样本相似的肠道形态。DON 诱导组织学评分显著降低，这是由于中度至重度的组织学变化（顶端坏死、绒毛萎缩和融合）以及形态计量学参数（绒毛高度、隐窝深度、绒毛高度：隐窝深度比和杯状细胞密度）显著降低所致。暴露于 COS+DON 的样本的肠道形态仍与 DON 处理相似。综上所述，低水平的 COS 不能对抗 DON 诱导的肠道病变。
