Babiuk L A, Ohmann H B, Gifford G, Czarniecki C W, Scialli V T, Hamilton E B
J Gen Virol. 1985 Nov;66 ( Pt 11):2383-94. doi: 10.1099/0022-1317-66-11-2383.
Treatment of calves with bovine recombinant alpha 1 interferon prior to challenge with bovine herpesvirus type 1 increased the animals' ability to withstand a subsequent Pasteurella haemolytica challenge. The reduction in viral-bacterial synergy observed following interferon treatment did not appear to be due to a direct effect of the interferon on virus replication in the upper respiratory tract. Thus, even though interferon-treated animals shed slightly less virus from their nasal passages than did untreated animals, this reduction was not statistically significant. Furthermore, there was no difference in the level of intranasal interferon secreted by control or interferon-treated animals. These results suggest that interferon treatment does not affect the production of endogenous interferon. In contrast, a significant difference was observed between the number of days that control animals were sick, the levels of serum fibrinogen and the functional activity of polymorphonuclear neutrophilic granulocytes obtained from infected calves. These results suggest that bovine recombinant alpha 1 interferon may have a greater immunomodulatory effect than a direct antiviral effect in this model. This is further supported by the observation that bovine herpesvirus type 1 is relatively resistant to the direct antiviral effect of bovine recombinant alpha 1 interferon in vitro.
在给小牛用1型牛疱疹病毒攻击之前，用牛重组α1干扰素进行处理，可提高动物抵御随后溶血巴斯德氏菌攻击的能力。干扰素处理后观察到的病毒-细菌协同作用的降低，似乎并非由于干扰素对上呼吸道病毒复制的直接作用。因此，尽管经干扰素处理的动物鼻腔排出的病毒略少于未处理的动物，但这种减少在统计学上并不显著。此外，对照动物或经干扰素处理的动物分泌的鼻内干扰素水平没有差异。这些结果表明，干扰素处理不影响内源性干扰素的产生。相比之下，在对照动物患病天数、血清纤维蛋白原水平以及从感染小牛获得的多形核嗜中性粒细胞的功能活性方面，观察到了显著差异。这些结果表明，在该模型中，牛重组α1干扰素可能具有比直接抗病毒作用更大的免疫调节作用。1型牛疱疹病毒在体外对牛重组α1干扰素的直接抗病毒作用相对耐药这一观察结果进一步支持了这一点。
