从人鼻病毒14型的cDNA克隆体外合成感染性RNA。
In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.
作者信息
Mizutani S, Colonno R J
出版信息
J Virol. 1985 Nov;56(2):628-32. doi: 10.1128/JVI.56.2.628-632.1985.
Abstract
Development of a novel infectious cDNA assay is described for human rhinovirus type 14. A full-length cDNA clone of the human rhinovirus type 14 genome RNA was assembled and transcribed in vitro by using the SP6 transcription system. Transfection of HeLa cells with the nascent RNA resulted in the production of rhinovirus indistinguishable from the parental virus by both immunological and polyacrylamide gel analysis.
摘要
本文描述了一种针对14型人鼻病毒的新型感染性cDNA检测方法的开发。通过使用SP6转录系统，组装了14型人鼻病毒基因组RNA的全长cDNA克隆并进行体外转录。用新生RNA转染HeLa细胞，通过免疫和聚丙烯酰胺凝胶分析，产生了与亲代病毒无法区分的鼻病毒。
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