Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering , Yancheng Teachers University , Yancheng 224051 , People's Republic of China.
School of Pharmaceutical Sciences , Wenzhou Medical University , Wenzhou 325035 , People's Republic of China.
J Agric Food Chem. 2018 Aug 1;66(30):8132-8141. doi: 10.1021/acs.jafc.8b01645. Epub 2018 Jul 18.
The growth and development of the Chinese oak silkworm, Antheraea pernyi, are strongly influenced by environmental conditions, including heavy metal pollution. An excess of heavy metals causes cellular damage through the production of free radical reactive oxygen species. In this study, transcriptome analysis was performed to investigate global gene expression when A. pernyi was exposed to zinc infection. With RNA sequencing (RNA-Seq), a total of 25 795 510 and 38 158 855 clean reads were obtained from zinc-treated and control fat body libraries, respectively. We identified 2399 differential expression genes (DEGs) (1845 upregulated and 544 downregulated genes) in the zinc-treated library. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these DEGs were related to the peroxisome pathway that was associated with antioxidant defense. Our results suggest that fat bodies of A. pernyi constitute a strong antioxidant defense against heavy metal contamination.
中国橡树蚕（Antheraea pernyi）的生长和发育受到环境条件的强烈影响，包括重金属污染。过量的重金属会通过产生自由基活性氧物质而导致细胞损伤。在这项研究中，进行了转录组分析，以研究 A. pernyi 暴露于锌感染时的全局基因表达。通过 RNA 测序（RNA-Seq），分别从锌处理和对照脂肪体文库中获得了 25795510 和 38158855 个清洁读数。我们在锌处理文库中鉴定了 2399 个差异表达基因（DEGs）（1845 个上调和 544 个下调基因）。此外，京都基因与基因组百科全书（KEGG）富集分析表明，这些 DEGs 与过氧化物酶体途径有关，该途径与抗氧化防御有关。我们的结果表明，A. pernyi 的脂肪体构成了对重金属污染的强大抗氧化防御。
