Zhang Lei, Zeng Yan, Qi Ji, Xu Yanxiao, Zhang Shaoqun, Zhou Xin, Ping Ruiyue, Fu Shijie
Orthopedics Department, Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan 646600, P.R. China.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Exp Ther Med. 2018 Jul;16(1):113-120. doi: 10.3892/etm.2018.6143. Epub 2018 May 10.
Cardio-cerebrovascular disease is one of the three major causes of mortality in humans and constitutes a major socioeconomic burden. Carotid atherosclerosis (CAS) is a very common lesion of the arterial walls, which leads to narrowing of the arteries, in some cases occluding them entirely, increasing the risk of cardiovascular events. The aim of the present study was to evaluate a cynomolgus monkey model of carotid atherosclerosis (CAS) induced by puncturing and scratching combined with a high-fat diet. A total of 12 cynomolgus monkeys were randomly divided into four groups: A, puncturing and scratching carotid artery intimas + high-fat diet (n=3); B, puncturing and scratching carotid artery intimas + regular diet (n=3); C, high-fat diet only (n=3); and D, regular diet only (n=3). Blood was harvested at weeks 4, 6 and 8 and plasma lipid levels were assessed. At week 8, monkeys were sacrificed and carotid arteries were harvested for hematoxylin and eosin (H&E) staining to observe pathological changes. The results revealed that a high-fat diet led to increased plasma lipid levels and accelerated plaque formation. Carotid color Doppler ultrasonography was performed and, along with H&E staining, revealed plaque formation in group A. In summary, the results of the present study suggest that a cynomolgus monkey model of CAS model may be successfully constructed by puncturing and scratching of the carotid artery intimas in combination with a high-fat diet.
心脑血管疾病是人类三大死因之一，构成了重大的社会经济负担。颈动脉粥样硬化（CAS）是动脉壁非常常见的病变，会导致动脉狭窄，在某些情况下会完全堵塞动脉，增加心血管事件的风险。本研究的目的是评估通过穿刺和刮擦结合高脂饮食诱导的食蟹猴颈动脉粥样硬化（CAS）模型。总共12只食蟹猴被随机分为四组：A组，穿刺和刮擦颈动脉内膜+高脂饮食（n = 3）；B组，穿刺和刮擦颈动脉内膜+常规饮食（n = 3）；C组，仅高脂饮食（n = 3）；D组，仅常规饮食（n = 3）。在第4、6和8周采集血液并评估血浆脂质水平。在第8周，处死猴子并采集颈动脉进行苏木精和伊红（H&E）染色以观察病理变化。结果显示，高脂饮食导致血浆脂质水平升高并加速斑块形成。进行了颈动脉彩色多普勒超声检查，与H&E染色一起显示A组有斑块形成。总之，本研究结果表明，通过穿刺和刮擦颈动脉内膜并结合高脂饮食，可能成功构建食蟹猴CAS模型。
