Gan Jing-Yun, Li Qing-Song, Zhou Huan-Ming, Zhang Wei, Lian Ling-Zhi, Yu Zhang, Zhang Zhen-Yong
Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
Department of Pathology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
Int J Ophthalmol. 2018 Jun 18;11(6):899-904. doi: 10.18240/ijo.2018.06.01. eCollection 2018.
To explore a feasible method on the establishment of an animal model of conjunctivochalasis (CCH).
Twelve clean-grade New Zealand white rabbits were divided into four groups (=3/group): the control group (one received no interventions, and the others underwent subconjunctival injection of sterile water), the matrix metalloproteinases (MMPs) group (administered subconjunctival injection of MMP-3), the aging group (administered subcutaneous injection of D-galactose), the tumor necrosis factor-α (TNF-α) solution group (administered eye drops of TNF-α). Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy (TEM) were performed after 12wk.
Among all groups, the MMPs group had the following changes: the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers (Trichrome staining) and the broken elastic fibers (Aldehyde-fuchsin staining); the focal necrosis of fibroblasts (TEM).
Administration of MMPs may be a feasible method for the establishment of an animal model of CCH.
探索一种建立结膜松弛症（CCH）动物模型的可行方法。
将12只清洁级新西兰白兔分为四组（每组 = 3只）：对照组（1只不接受干预，其余接受结膜下注射无菌水）、基质金属蛋白酶（MMPs）组（结膜下注射MMP - 3）、衰老组（皮下注射D - 半乳糖）、肿瘤坏死因子 - α（TNF - α）溶液组（滴注TNF - α滴眼液）。12周后进行眼前节照相、结膜组织光学显微镜检查和透射电子显微镜（TEM）检查。
在所有组中，MMPs组有以下变化：下睑结膜与巩膜之间连接更疏松；胶原纤维更紊乱（三色染色）和弹性纤维断裂（醛复红染色）；成纤维细胞灶性坏死（TEM）。
给予基质金属蛋白酶可能是建立结膜松弛症动物模型的一种可行方法。
